Adverse Cell Culture Conditions Mimicking the Tumor Microenvironment Upregulate ABCG2 to Mediate Multidrug Resistance and a More Malignant Phenotype

Table 1

Effect of a 16-h pretreatment of 2-DG (20 mM) or hypoxia on reducing the cytotoxic effect of ABCG2 substrate anticancer drug in HCT-116, S1 and S1M1-80.

Drug		IC50±SD
	HCT-116	S1	S1M1-80
Mitoxantrone (μM)
Mitoxantrone alone	4.2±1.2	0.15±0.03	48±2.3
with 2-DG	9.6±1.5^∗	0.48±0.04^∗	80±4.1^∗
in Hypoxia	10.1±1.3^∗	0.55±0.04^∗	92±7.9^∗
+FTC (5 μM)	5.1±1.1	0.13±0.02	0.25±0.10^∗
Cisplatin (μg/mL)
Cisplatin alone	8.3±1.1	2.4±0.9	3.3±1.1
with 2-DG	6.5±1.3	3.1±1.0	4.9±0.6
in Hypoxia	8.7±1.6	3.5±1.2	4.3±1.3
+FTC (5 μM)	9.2±0.8	2.1±0.8	4.6±0.8

^∗𝑃<0.05 , versus IC₅₀ of the anticancer drug alone.