Research Article

A Novel Membrane Protein-Specific Serine/Threonine Kinase: Tissue Distribution and Role in Sperm Maturation

Figure 2

Immunoassay of CIK in vertically motile sperm and weakly motile/nonmotile sperm plasma membrane: spermatozoa from the cauda epididymis were suspended in modified Ringer solution containing 2% Ficoll and were placed at the bottom of a beaker containing modified Ringer’s solution. Vertically motile sperms were then collected by swim up technique. Sperm plasma membranes were prepared as described in Section 2. Distribution of CIK was determined by ELISA under standard assay condition. The data shown are Mean ± s.e.m. of 5 experiments.
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