Figure 5: Pattern of Akt phosphorylation in PC12 and IMR32 cells exposed to NGF in the presence or absence of U0126. Cells were pretreated with inhibitor 1 h prior to treatment with NGF. (a) Representative immunoblots of lysates obtained from PC12 or IMR32 cells grown in the presences of 50 μM U0126 following treatment with NGF (50 ng/mL) for 15 to 240 min. Phosphorylated and nonphosphorylated forms of Akt are identified by the primary antibody indicated above each panel. Densitometric analysis of phosphorylated Akt normalized to total Akt levels in PC12 (b) and IMR32 (c) cells treated with NGF in the presence or absence of U0126. NGF treatment significantly increased Akt phosphorylation above control levels in the presence or absence of U0126 (**, ***). This figure is representative of immunoblots obtained from 6 independent experiments.