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ISRN Spectroscopy
Volume 2012 (2012), Article ID 917879, 6 pages
http://dx.doi.org/10.5402/2012/917879
Research Article

Quick Multicomponent Analysis Based on Matrix Isopotential Synchronous Fluorimetry

Department of Experimental Physics, University of Pécs, Ifjúság u. 6., Pécs 7624, Hungary

Received 5 November 2012; Accepted 21 November 2012

Academic Editors: M. L. Fernández de Córdova, L. Charbonnière, and S. Pandey

Copyright © 2012 Géza Makkai and János Erostyák. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Fast and precise methods are important in chemical and physical analysis, but determination of concentration of selected components in mixtures may have special difficulties. Excitation-emission matrices (EEM) are widely used in characterizing fluorescence emission. Based on these primary data, we present an improvement of matrix isopotential synchronous fluorimetry (MISF) and a method which ensures a very fast multicomponent analysis. MISF is generally combined with derivative technique (DMISF) to be able to eliminate the contribution of the background emission. Unfortunately, it is too sensitive to noises and fluctuations; thus the concentration region where it can be used, was limited until now. Our first aim was to reduce the influence of measurement errors and to increase the sensitivity of known MISF method by more than one order of magnitude. As a further result, using improved MISF, we got a method which ensures very fast determination of the wanted compound's concentration both in presence of one- or even two-component background of unknown concentration.