Figure 2: Flow cytometry analysis of draining LN 6, 9, and 12 days after B16F10 and PBS injection. Lymph nodes cells were stained with antibodies for B220, CD8, CD4, and CD11c, and CD86 (in CD11c+ cells). (a) Total lymph node cells were counted. (b)–(e) Total number of positive cells for each staining (f) Mean CD86 fluorescence in CD11c+ cells. Tumor, (○) and PBS, (■) lymph nodes. Values of were considered statistically significant. Data representative of three different experiments, .