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Figure 6: Antigen presentation and TEa differentiation in tumor draining lymph node (DLN) with LPS injection. Mice were adoptively transferred with 5 × 1 0 4 TEa cells and 24 h later injected with 5 × 1 0 5 B16EaRed cells or PBS. On day 5, some mice received intratumoral LPS injection and 48 h later the DLN were excised and analyzed by FACS. (a) Mean absolute number of YAe+, CD86+ cells in DLN (gated on CD11c+, B220 cells). (b) Dot plot showing CD11c+, B220 cells. (c) Absolute numbers of INF- producing cells (gated on CD4+, CD90.1+ cells). (d) Dot plot showing CD4+, CD90.1+ cells. (e) Dot plot showing cells CD4+, CD90.1+ IFNγ + cells. Numbers in the plots represent mean percentages in gates. Data representative of two different experiments, 𝑁 = 3 mice per group. ( 𝑃 < 0 . 0 5 ).