VEGF Is Involved in the Increase of Dermal Microvascular Permeability Induced by Tryptase

Figure 1

Effect of tryptase and HMC-1 supernatant on the permeability of HDMECs. As described in Methods, the permeability of HDMECs after indicated treatment was detected by measuring fluorescence in the lower chamber at 490 nm after incubation with FITC-dextran for 1 h in the upper chamber. The changes of OD490 in the lower chamber after 1 h of incubation were calculated for the permeability of HDMECs. (a) Effect of tryptase at different concentrations on the permeability of HDMECs in the presence or absence of APC366. The heparin was used as Vehicle control. (b) Effect of HMC-1 supernatant at different concentrations on the permeability of HDMECs with or without APC366. (c) Effect of anti-VEGF antibody and SU5416 on the increase of permeability stimulated by tryptase. (d) Effect of anti-VEGF antibody on the increase of permeability stimulated by HMC-1 supernatant. ^∗𝑃<0.05 compared to the group of nonaddition. ^#𝑃<0.05 compared to the group only treated with tryptase. ^&𝑃<0.05 compared to the group only treated with HMC-1 supernatant.

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