VEGF Is Involved in the Increase of Dermal Microvascular Permeability Induced by Tryptase

Table 1

Primer pairs used to amplify PCR products.

Gene	Sequence (5′-3′)	Product size	Annealing T (°C)	GeneBank no.
VEGF	Forward: CAACATCACCATGCAGATTATGC	132 bp	60°C	NM_001033756
	Reverse: CCCACAGGGATTTTCTTGTCTT
Flt-1	Forward: TGGCTGCGACTCTCTTCTG	118 bp	60°C	NM_002019
	Reverse: CAAAGGAACTTCATCTGGGTCC
Flk-1	Forward: GGCCCAATAATCAGAGTGGCA	104 bp	60°C	NM_002253
	Reverse: TGTCATTTCCGATCACTTTTGGA
GAPDH	Forward: CATGAGAAGTATGACAACAGCCT	113 bp	60°C	NM_002046
	Reverse: AGTCCTTCCACGATACCAAAGT