Research Article
Affinity and Matrix Effects in Measuring Fish Plasma Vitellogenin Using Immunosorbent Assays: Considerations for Aquatic Toxicologists
Table 1
The effects of dilution medium on measured concentrations of vitellogenin (mean ± standard error, (sample size)). Plasma from three fathead minnows was analyzed via competitive ELISA using either polyclonal or monoclonal antibody and quantified in one of three separate standard curves prepared in different matrices. Normal method: prepared in phosphate-based dilution buffer. Diluted plasma: unexposed plasma substituted for dilution buffer, where the plasma was diluted with the standards across a range of 1 : 16 to 1 : 1024. Constant plasma: unexposed plasma substituted for dilution buffer with standards added, where the plasma was diluted 1 : 500. Variability in number of samples analyzed reflects sample values outside the linear range of standard curve.
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