Methylenetetrahydrofolate Reductase C677T and A1298C Mutations in Women with Recurrent Spontaneous Abortions in the Northwest of Iran

Table 1

Sequence of primers, PCR product size, restriction enzymes, and size of digested fragments that are used for screening by PCR-RFLP.

Mutation	Sequence of primers	PCR product	Restriction	Wild type	Heterozygote	Mutant
		(bp)	enzyme
MTHFR	F-TGAAGGAGAAGGTGTCTGCGGGA	198	Hinf1	198	198/175/23	175/23
C677T	R-AGGACGGTGCGGTGAGAGTG
MTHFR	F-CTTTGGGGAGCTGAAGGACTACTAC	163	MboII	56/31/30/28/18	84/56/31/30/28/18	84/31/30/18
A1298C	R-CACTTTGTGACCATTCCGGTTTG