Immunomodulatory Effects of Danshen (Salvia miltiorrhiza) in BALB/c Mice
Table 3
Danshen effects on the numbers of peripheral blood leukocyte subpopulations before and after LM infection.
Danshen (% of daily diet)
Neutrophils (cells/L)
Monocytes (cells/L)
Lymphocytes (cells/L)
NK cells (cells/L)
Preinfection
Postinfection
P value
Preinfection
Postinfection
P value
Preinfection
Postinfection
P value
Preinfection
Postinfection
P value
0
2747 ± 279
2038 ± 908
ns
717 ± 146
885 ± 371
ns
4865 ± 694
1258 ± 523
0.018
193 ± 32
214 ± 57
ns
0.5
1903 ± 147
2928 ± 1462
ns
798 ± 95
1213 ± 425
ns
4509 ± 594
1841 ± 508
0.014
136 ± 26
653 ± 118
0.029
1
3022 ± 890
2128 ± 399
ns
912 ± 134
1051 ± 134
ns
4848 ± 737
1242 ± 246
0.004
219 ± 82
543 ± 191
ns
2
2749 ± 1344
2414 ± 247
ns
484 ± 71
1220 ± 253
0.012
3419 ± 518
1890 ± 460
ns
104 ± 22
321 ± 70
0.006
After ingesting 0–2% Danshen for 70 days, peripheral blood was collected before and after LM infection. The sample was prepared in a TruCOUNT tube and analyzed by flow cytometry. The absolute number of neutrophils, monocytes, lymphocytes, and NK cells was calculated based on four to nine mice from 2–4 separated experiments. Data are presented as mean ± SEM.
