Innate Immunity in Lobsters: Partial Purification and Characterization of a Panulirus cygnus Anti-A Lectin

Table 3

Inhibition of haemagglutination by P. cygnus lectin with sugars. Minimum inhibitory concentration (mmol/L)^a.

Sugar	Haemolymph	Purified lectin^b
N-acetylgalactosamine	12.5	12.5
N-acetylglucosamine	12.5	6.2
β-methylgalactoside	50	50

^aThe minimum inhibitory concentration is the lowest concentration of sugar that completely inhibited HA with rat erythrocytes for haemolymph or purified lectin diluted to contain 5 rat erythrocyte-HA units in 25 mL.
^bPurified lectin was obtained by affinity chromatography on REM-Sepharose, with sucrose-urea as eluant. The lectin was diluted 1 : 100 to give 5 HA units for inhibition tests. The A₂₈₀ of the undiluted lectin preparation was 0.2.