Research Article
A Survey for Escherichia coli Virulence Factors in Asymptomatic Free-Ranging Parrots
Table 2
Virulence factors and gene sequences tested by polymerase chain reaction (PCR) according to the described references.
| | Virulence factor | Sequence (5′–3′) | Reference |
| | Intimin (eae) | F-CTGAACGGCGATTACGCGAA |
Aranda et al. [26] | | R-CGAGACGATACGATCCAG | | Bundle forming pili (bfp) | F-AATGGTGCTTGCGCTTGCTGC | Aranda et al. [26] | | R-GCCGCTTTATCCAACCTGGTA | | S fimbrial adhesin (sfa) | F-CTCCGGAGAACTGGGTGCATCTTAC | Yamamoto et al. [27] | | R-CGGAGGAGTAAT TACAAACCT GGCA | | P fimbrial adhesin (pap) | F-GCAACAGCAACGCTGGTTGCATCAT | Yamamoto et al. [27] | | R-AGAGAGAGCCACTCTTATACGGACA | | Increased serum survival (iss) | F-AGAGAGAGCCACTCTTATACGGACA | Ewers et al. [28] | | R-CTATTGTGAGCAATATACA | | Aerobactin (iuc) | F-TACCGGATTGTCATATGCAGACCGT | Yamamoto et al. [27] | | R-AATATCTTCCTCCAGTCCGGAGAAG | | Temperature-sensitive hemagglutinin (tsh) | F-ACTATTCTCTGCAGGAAGTC | Ewers et al. [28] | | R-CTTCCGATGTTCTGAACGT | | Alpha hemolysin (hlyA) | F-AACAAGGATAAGCACTGTTCTGGCT | Yamamoto et al. [27] | | R-ACCATATAAGCGGTCATTCCCGTCA | | Cytotoxic necrotizing factor (cnf1) | F-AAGATGGAGTTTCCTATGCAGGAG | Yamamoto et al. [27] | | R-CATTCAGAGTCCTGCCCTCATTATT |
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