Increased Resistance of Breast, Prostate, and Embryonic Carcinoma Cells against Herpes Simplex Virus in Three-Dimensional Cultures
Figure 1
Morphology of MCF-10A, MB-231, PC-3, and P19 grown under 2D and 3D culture conditions, 5 to 6 days after establishment of the cultures. MCF-10A, an immortalized nontumorigenic breast epithelial cell line, shows epithelial morphology in 2D cultures (a), while these cells form adenoid-like, compact aggregates on the surface of the Matrigel matrix in 3D cultures without evidence of cells invading the matrix (b). MB-231 invasive breast cancer cells show bi- and tripolar morphology in 2D cultures (c). MB-231 cells growing under 3D culture conditions form monolayers and loose cell aggregates on the surface of the matrix. From these aggregates, individual cells invade the Matrigel matrix (d). PC-3 prostate cancer cells show epithelial morphology in 2D cultures (e). When placed on Matrigel surface, PC-3 cells form aggregates, from which individual cells invade into the matrix, similarly to but less extensively as MB-231 cells (f). Embryonal teratocarcinoma-derived P19 cells also have epithelial morphology in conventional 2D cultures (g), while under 3D culture conditions cells form tube-like structures reminiscent of vasculogenic mimicry patterns (h). Magnification: 100x.
