Increased Resistance of Breast, Prostate, and Embryonic Carcinoma Cells against Herpes Simplex Virus in Three-Dimensional Cultures
Figure 4
Morphology (a, c, e, and g) and GFP expression indicative of HSV-1 infection (b, d, f, and h) in three-dimensional MCF-10A, MB-231, PC-3, and P19 cultures 2 to 3 days after HSV-1 K26GFP inoculation. Most of MCF-10A cell aggregates on the Matrigel surface emitted green fluorescent light (b) consistent with extensive HSV-1 replication. Most of MB-231 cell aggregates on the Matrigel surface also expressed GFP (d) consistent with extensive HSV-1 replication. However, MB-231 cells invading the Matrigel matrix were often negative (d). Similarly, PC-3 cell aggregates on the matrix surface were extensively GFP-positive, while many invading cells were negative (f). Three-dimensional P19 cultures showed no to very limited GFP expression (h). Magnification: 100x.
