Increased Resistance of Breast, Prostate, and Embryonic Carcinoma Cells against Herpes Simplex Virus in Three-Dimensional Cultures

Table 2

HSV-1 replication (GFP expression) in MCF-10A, MB-231, PC-3, and P19 cells first exposed to HSV-1 K26GFP for 1 hour under 2D conditions to ensure exposure of cells to virus and then cultured for 1 day either under 2D or 3D conditions.

Cell type	Culture type	% of cells GFP-positive at day 1*
		MOI = 0.01	MOI = 1
MCF-10A	2D	ND	90
	3D	ND	80
MB-231	2D	0.5	80
	3D	0.1	60
PC-3	2D	2	70
	3D	0.4	50
P19	2D	0.2	40
	3D	0.02	30

∗Percentage of GFP-expressing cells was determined by counting the number of GFP-expressing and GFP-negative cells in 16 high power microscopic fields for each studied type of treatment at two different multiplicities of infection (MOI = 0.01 and 1 PFU per cell). ND: not done.