Mouse Models and Techniques for the Isolation of the Diabetic Endothelium
Figure 4
Confirmation of endothelial identity. GFP+ cells sorted by FACS were collected directly into media. (a) Cells were spun onto slides and fixed for viewing by fluorescence microscopy to confirm the presence of only GFP+ cells. (b) Sorted cells form tubular structures after 5 days when plated on matrigel. (c) Sorted cells cultured for 5 days take up Dil-Ac-LDL after a 4 h incubation.
