Mouse Models and Techniques for the Isolation of the Diabetic Endothelium

Table 2

Weights and fasting glucose levels of Tie2-GFP, ScN/GFP, and ApoE(−/−)/GFP mice receiving a high-fat diet versus chow diet.

Weeks	Tie2-GFP		ScN/GFP		ApoE(−/−)/GFP
on diet	Chow	HFD	Chow	HFD	Chow	HFD
Weight (g)
2	24.1 ± 0.8 (12)	30.0 ± 1.6 (14)**	20.5 ± 0.4 (4)^†	24.9 ± 0.9 (5)^**∧	23.1 ± 2.0 (3)	31.5 ± 0.8 (7)*
4	26.8 ± 0.8 (18)	31.1 ± 1.3 (19)**	22.7 ± 0.8 (7)^‡	26.7 ± 1.1 (7)^**∧	26.6 ± 0.9 (13)	33.0 ± 0.9 (16)***
6	26.3 ± 0.8 (14)	33.8 ± 1.2 (15)***	25.5 ± 0.8 (12)	28.4 ± 0.6 (12)^**‡	27.7 ± 1.2 (11)	34.7 ± 1.2 (12)***
8	28.0 ± 0.9 (14)	36.5 ± 1.0 (15)***	24.0 ± 0.3 (3)^‡	33.0 ± 1.5 (3)*	25.2 ± 1.7 (8)	33.7 ± 2.3 (7)*
Glucose (mg/dL)
2	148 ± 9 (12)	190 ± 10 (14)**	85 ± 7 (4)^‡	117 ± 11 (5)^*‡	152 ± 17 (3)	168 ± 19 (7)
4	116 ± 8 (16)	163 ± 9 (17)***	82 ± 14 (3)	131 ± 9 (7)^*†	144 ± 8 (6)^∧	184 ± 13 (10)*
6	139 ± 9 (18)	210 ± 10 (18)***	85 ± 5 (10)^‡	118 ± 9 (10)^**‡	137 ± 18 (7)	157 ± 10 (7)^†
8	111 ± 4 (11)	182 ± 4 (9)***	76 ± 15 (5)	137 ± 5 (5)^**‡	160 ± 2 (3)^‡	212 ± 3 (3)^***‡

Data are means ± SE. Weight measurements and glucometry of the tail blood were performed after 2, 4, 6, and 8 weeks of feeding. 𝑁’s are indicated in parentheses.*𝑃<0.05, **𝑃≤0.01, and ***𝑃<0.001 versus chow-fed controls; ^∧𝑃<0.05, ^†𝑃≤0.01, and ^‡𝑃<0.001 versus Tie2-GFP.