Mouse Models and Techniques for the Isolation of the Diabetic Endothelium

Table 3

Flow cytometry analysis of Tie2-GFP expression in peripheral blood mononuclear cells (PBMCs) derived from Tie2-GFP mice fed a high-fat or chow diet for 8 weeks.

	CD11b^+ (% of CD45^+)	CD11b^+/CD115^+ (% of CD45^+)	GFP^+ (% of CD45^+/CD11b^+)	GFP^+ (% of CD45^+/CD11b^+/CD115^+)
Chow	30 ± 4	9 ± 2	0.6 ± 0.1	0.8 ± 0.3
HFD	33 ± 6	11 ± 3	0.6 ± 0.2	0.8 ± 0.5

PBMCs were isolated and stained for CD45, CD11b, and CD115. The population positive for the common leukocyte antigen, CD45, was analyzed for CD11b⁺ myeloid cells and CD11b⁺/CD115⁺ monocytes. The percentage of these populations appearing to have a positive signal for GFP is shown. Data is presented as mean ± SD (𝑛=3).