Linen Most Useful: Perspectives on Structure, Chemistry, and Enzymes for Retting Flax

Table 6

Fiber separation and strength by purified enzymes.

Enzyme formulation^1	Fried test score	Strength
Endopolygalacturonase (EPG)	2.3 ± 0.4	29.7 ± 3.3
EPG + xylanase (xyl)	2.4 ± 0.5	29.8 ± 5.3
EPG + endoglucanase (EG)	2.3 ± 0.5	26.9 ± 3.7
EPG + Xyl + EG	2.0 ± 0	22.3 ± 1.6
Xyl	1.1 ± 0.2	ND
EG	1.4 ± 0.5	ND
EPG + Xyl +EG + Celluclast	2.4 ± 0.2	ND

¹EPG is endopolygalacturonase purified from Rhizopus oryzae sb (NRRL 29086) used at 18.1 U/mL to equal pectinase activity in SP 240; Xyl is a cloned xylanase from Thermatoga maritima [29]; EG is endoglucanase Novozymes SP 613; Celluclast is a Novozymes cellulase from Trichoderma reesei ATCC26921. All enzyme mixtures contained 50 mmol oxalic acid as chelator.
Data from [30].