Spectrophotometric Determination of Some Non-Sedating Antihistamines Using Erythrosine B

Table 4

Robustness of the developed spectrophotometric method.

Drug	% Recovery* ± SD
	CTZ	EBS	FXD	KET	LOR
No variation**	101.1±0.76	100.4±0.45	100.2±0.93	100.9±1.29	100.2±1.20
pH of acetate buffer
3.2	101.7±0.82	100.7±1.16	100.6±1.07	100.9±0.61	100.3±0.52
3.4	100.9±0.49	100.9±0.27	101.3±0.72	102.3±1.22	97.4±0.01
Acetate buffer conc.
0.15 M	100.8±0.57	101.0±0.73	100.2±0.63	99.6±1.12	99.4±0.84
0.25 M	101.7±0.08	101.2±0.56	101.4±0.02	101.8±0.80	98.7±0.91
Erythrosine B conc.
0.14% w/v	100.1±1.14	99.3±0.97	100.2±1.11	98.1±0.44	99.4±0.67
0.16% w/v	102.1±0.77	98.2±0.63	100.8±0.39	100.7±0.36	100.6±1.02
Erythrosine B volume
0.44 mL	101.9±0.43	98.5±0.89	100.3±0.55	98.6±0.32	102.6±1.11
0.46 mL	102.3±0.37	102.3±1.57	102.5±0.22	103.0±0.54	102.8±0.66
Reaction time
8 min.	101.6±0.15	98.5±1.03	100.9±0.83	102.1±0.69	99.1±1.11
10 min.	98.8±1.8	102.9±0.42	99.6±0.19	98.9±1.29	98.3±0.82

∗Average of six replicates.
**No variation in the assay condition of the proposed method.