Identification of PDZ Domain Containing Proteins Interacting with 1.2 and PMCA4b
Figure 5
Colocalization of Cav1.2 with NHERF1 and MAGI-3, and PMCA4b with MAGI-3 in rat cardiomyocytes. Double immunofluorescent staining of Cav1.2 and NHERF1 ((a)–(c)), MAGI-3 and Cav1.2 ((d)–(f)), and PMCA4b and MAGI-3 ((g)–(i)) in rat cardiomyocytes. For heart sections the following antibodies were used: polyclonal rabbit anti-Cav1.2-ATTO 488 ((a), (d)), polyclonal rabbit anti-NHERF1 (b), polyclonal rabbit anti-MAGI-3 ((e), (g)), and monoclonal mouse anti-PMCA 5F10 (h) followed by Alexa Fluor 488 goat anti-mouse, or Alexa Fluor 594 goat anti-rabbit, where appropriate. (c), (f), and (i) are merged images. Cav1.2 and NHERF1 are coexpressed at the intercalated discs of cardiomyocytes (see arrow ), Cav1.2 and MAGI-3 at the intercalated discs and transverse tubules (T-tubuli) (see arrow ). PMCA4b and MAGI-3 are located at the T-tubules as well (see arrow ).
