Identification of PDZ Domain Containing Proteins Interacting with 1.2 and PMCA4b
Figure 7
Electrophysiological properties of Ba2+ currents from Cav1.2 subunits. (a) Pattern of electrical stimulation. The membrane potential was clamped at a holding potential of −70 mV. From the holding potential the cells were depolarized by nine voltage steps with +10 mV incremental amplitudes and 50 ms duration. (b) currents induced by the electrical stimulation shown in (a) in a cell expressing wild-type Cav1.2 channels. (c) Cav1.2 channel Ba2+ currents induced by the electrical stimulation shown in (a) in a cell expressing nNOS. (d) Maximal current density of control Cav1.2 currents, Cav1.2 subunits in the presence of nNOS. (e) Voltage dependence of Ba2+ currents: currents were normalized to the maximal current amplitude and plotted against the potentials of the electrical stimulation; the curve was fitted using the Boltzmann equation. (f) Activation threshold of Ba2+ currents from Cav1.2 subunits and Cav1.2 subunits in the presence of nNOS; the numbers indicate the level of significance. (g) Voltage of half-maximal activation currents from Cav1.2 subunits and Cav1.2 subunits in the presence of nNOS; the potentials of half-maximal activation were significantly shifted towards positive potentials in the presence of nNOS. (h) Slope of Boltzmann curve () of currents from Cav1.2 subunits and Cav1.2 subunits in the presence of nNOS; the values were significantly larger in the presence of nNOS. (i) Comparison of the voltages of maximal current amplitudes (); in the presence of nNOS was shifted towards more positive voltages of currents from Cav1.2 subunits.
