Research Article
A Sequence-Specific Nicking Endonuclease from Streptomyces: Purification, Physical and Catalytic Properties
Table 1
Summary of the purification of the nicking endonuclease from Streptomyces.
| | Step | Volume (mL) | Protein concentration (mg/mL) | Total protein (mg) | Specific activity (U/mg) | Total units (U) | Fold purification (x) | Recovery (%) |
| | Cell-free extract | 57 | 11.6 | 661 | 8.62 | 5,700 | 1.0 | 100 | | 0–60% NH3SO4 | 20 | 18.5 | 370 | 10.8 | 4,000 | 1.25 | 70 | | DEAE cellulose | 3 | 5.5 | 16.5 | 54.5 | 900 | 6.0 | 16 | | Phenyl-Sepharose | 1 | 0.2 | 0.2 | 1000 | 200 | 116 | 2 |
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