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ISRN Biomarkers
Volume 2013 (2013), Article ID 316528, 5 pages
Research Article

Short-Term Stability of Biomarkers of Oxidative Stress and Antioxidant Status in Human Serum

1Center for Health Protection, National Institute of Public Health and the Environment, P.O. Box 1, 3720 BA Bilthoven, The Netherlands
2Department of Biochemistry, Medical Academy, Lithuanian University of Health Sciences, A. Mickeviciaus 9, 44307 Kaunas, Lithuania
3Department of Biological and Biochemical Sciences, Faculty of Chemical Technology, University of Pardubice, Studentska 573, 53210 Pardubice, Czech Republic
4Department of Clinical Biochemistry and Diagnostics, Regional Hospital of Pardubice, Kyjevska 44, 53203 Pardubice, Czech Republic

Received 16 April 2013; Accepted 26 May 2013

Academic Editors: Z. Feng and S. Paris-Palacios

Copyright © 2013 Eugène H. J. M. Jansen et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


The oxidation and antioxidant status of serum are often determined in serum samples which have been frozen for some time. The oxidative stress process is prone to fast alterations in the sample because of the possible instability of the reactants. Here one oxidation assay (ROM) and three antioxidant assays (FRAP, TAS, and BAP) have been tested on their performance and stability at short-time storage. The most commonly used temperatures for storage and handling of serum samples (+4 and +20°C) were selected. In three short-term studies in which the storage time varied between 3 and 48 hrs the performance of these assays were tested on human serum samples. The general conclusion is that most assays performed well and gave stable results during 2 days of storage of the samples at both temperatures. Only the FRAP and TAS assays showed a small deviation at some storage conditions. In conclusion, handling of serum samples at +4 and +20°C during short-time periods did not affect the quality and performance of the oxidation and antioxidant assays during day-to-day analyses.