Research Article

Amelogenin Peptide Extract Increases Differentiation and Angiogenic and Local Factor Production and Inhibits Apoptosis in Human Osteoblasts

Figure 5

Effect of rhAmelogenin and Fraction C on DNA content and osteoblast phenotype of human osteoblasts. Human osteoblasts were grown to confluence and then treated with 0, 1, or 10 μg/mL rhAmelogenin or Fraction C for 24 hours. DNA content (a) and alkaline phosphatase-specific activity (b) were measured in the cell lysate, and osteocalcin (c), osteoprotegerin (d), VEGF-A (e), and PGE2 (f) measured in the conditioned media. * , versus 0 μg/mL; , versus 1 μg/mL.
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