353598.fig.003a
(a) Cortex
353598.fig.003b
(b) Outer medulla 
353598.fig.003c
(c) Cortex
353598.fig.003d
(d) Outer medulla
Figure 3: Confirming CD133 upregulation in the injured renal proximal and distal tubules. KIM-1 is an injury marker, only positive in the injured proximal tubules [18]. With tubular injury, proximal tubules were diffusely positive for both CD133 (pink) and KIM-1 (brown) in the proximal tubules (*) of both cortex (a) and outer medulla (b), whereas CD133 (pink) was also positive in distal tubules (#) of both cortex (a) and outer medulla (b). To confirm CD133 positivity in the injured distal nephron tubules, we used cytokeratin 7 (CK7) staining as a distal tubular marker in (c) and (d). In addition to positive CD133 (brown) in the injured proximal tubules (*) from both cortex (c) and outer medulla (d), CD133 (brown) coexpressed with CK7 (pink) in the distal tubules (#) from both cortex (c) and outer medulla (d) (Magnification ×600, (a)–(d)).