Determination of the Binding Parameters between Proteins and Luminol by Chemiluminescence Using Flow Injection Technique

Figure 2

Relative CL intensity-time profile in different CL systems at 298 K. Curve 1: luminol CL system; Curve 2: luminol-BSA CL system; Curve 3: luminol-LYS CL system; Curve 4: luminol-MB CL system; Curve 5: luminol-CAT CL system. Concentration: luminol, 2.5 × 10⁻⁵ mol L⁻¹; BSA, LYS, MB, and CAT, 5.0 × 10⁻⁸ mol L⁻¹.