Determination of the Binding Parameters between Proteins and Luminol by Chemiluminescence Using Flow Injection Technique

Table 1

The linear equations and binding parameters by (10).

Protein	𝑇 (K)	Linear equation/𝑅; Δ𝐼=𝐴𝐶𝑝+𝐵^a	Linear range (nmol L^−1)	lg(𝐼0−𝐼)/𝐼 versus Lg[𝑃]
				𝐾𝑎 (L mol^−1)	𝑛
BSA	288	Δ𝐼=0.18𝐶BSA+29.91/0.9977	5–250	1.77 × 10^5	2.23
	298	Δ𝐼=0.19𝐶BSA+32.41/0.9963		2.34 × 10^5	1.75
	308	Δ𝐼=0.21𝐶BSA+35.57/0.9978		2.90 × 10^5	1.80
LYS	288	Δ𝐼=0.15𝐶LYS+21.49/0.9990	10–500	1.93 × 10^5	1.95
	298	Δ𝐼=0.21𝐶LYS+21.43/0.9961		4.26 × 10^5	1.75
	308	Δ𝐼=0.48𝐶LYS+22.02/0.9988		5.59 × 10^5	1.96
MB	288	Δ𝐼=0.19𝐶MB+32.61/0.9963	10–1000	2.12 × 10^6	1.26
	298	Δ𝐼=0.21𝐶MB+58.55/0.9966		2.40 × 10^6	1.35
	308	Δ𝐼=0.29𝐶MB+49.22/0.9961		2.93 × 10^6	0.94
CAT	288	Δ𝐼=4.91𝐶CAT+14.85/0.9962	0.1–100	4.38 × 10^7	1.38
	298	Δ𝐼=5.58𝐶CAT+15.74/0.9997		4.96 × 10^7	1.36
	308	Δ𝐼=6.14𝐶CAT+13.30/0.9996		5.93 × 10^7	1.24

aEach result is the average of seven separate determinations.