Research Article
Thermostable Alkaline Phytase from Alcaligenes sp. in Improving Bioavailability of Phosphorus in Animal Feed: In Vitro Analysis
Table 1
Summary of the purification of the phytase from Alcaligenes sp.
| | Purification step | Total activity (U) | Total protein (mg) | Specific activity (U/mg) | Purification (fold) | Yield (%) |
| | Crude enzyme | 980 | 182 | 5.38 | 1.0 | 100 | | 80% (NH4)2SO4 | 562 | 84.6 | 6.64 | 1.2 | 57.3 | | DEAE cellulose | 248 | 12.9 | 19.2 | 3.57 | 25.3 | | Sephadex G-75 | 69.3 | 2.8 | 25.7 | 4.75 | 7.07 |
|
|
