Simultaneous Extraction Optimization and Analysis of Flavonoids from the Flowers of Tabernaemontana heyneana by High Performance Liquid Chromatography Coupled to Diode Array Detector and Electron Spray Ionization/Mass Spectrometry

Table 5

Retention times (𝑅_𝑡), molecular ions [M + H]⁺, fragment ions, and relative abundance (%) of T. heyneana PTLC flower eluates (flavonols, flavonoid glycosides, and phenolic acids and derivatives scanned at 260 nm (PDA)).

S. no.	Retention time (𝑅𝑡, min)	MS m/z [M + H]^+	m/z fragment ions	Identified molecular weight	Identified compound	Relative abundance (%)
PTLC flower eluate 1
1	2.55	663	388/372/357	663	Unknown	100
2	3.54	340	184	340	Dicaffeic acid (diCA)	100
3	5.27	741	—	741	Kaempferol-3-O-robinoside-7-O-rhamnoside/robinin	100
PTLC flower eluate 2
4	3.19	383	224	383	Sinapic acid-hexoside (hydroxycinnamic acid sugar derivative)	100
5	3.36	525	—	525	Unknown	90
6	3.69	610	303	610	Rutin	100
PTLC flower eluate 3
7	3.17	303	—	303	Quercetin	55