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ISRN Biomarkers
Volume 2013 (2013), Article ID 474107, 5 pages
http://dx.doi.org/10.1155/2013/474107
Research Article

Microsatellite Instability in Head and Neck Squamous Cell Carcinoma: A Study of a Brazilian Population

1Núcleo de Genética Humana e Molecular, Departamento de Ciências Biológicas, Centro de Ciências Humanas e Naturais, Universidade Federal do Espírito Santo, Avenida Marechal Campos, 1468, Maruípe, 29043-900 Vitória, ES, Brazil
2Hospital Santa Rita de Cássia, Setor de Patologia, Vitória, ES, Brazil

Received 9 September 2013; Accepted 28 October 2013

Academic Editors: H.-L. Chan, A. Debucquoy, and M. Lotfy

Copyright © 2013 Elaine Stur et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Squamous cell carcinoma (SCC) is the sixth most common solid tumor in the world. Apart from known risk factors for head and neck SCC (HNSCC), there is a lack of information about genetic susceptibility regions that may play pivotal roles in the tumorigenesis of these tumors. Therefore, we have aimed to analyze the presence of genetic instability in microsatellite markers distributed in the genome. Microsatellite instability (MSI) was found in 6 HNSCC patients, among which only one was detected by the D17S250 marker, whereas the other 5 occurrences (13.5%) were detected by the D3S1611 marker. No instability was found at markers D5S346, D10S197, D11S922, and D11S988. MSI detected by D3S1611 marker was present in 3 (14.3%) moderately differentiated tumors and in 2 (25.0%) poorly differentiated tumors, but no statistical significance was found. Genotypic frequencies for all markers showed no statistically significant distribution alteration, neither were they related to differentiation grade or patient age. Marker D3S1611 is located in the MLH1 gene, which is part of the mismatch repair system (MMR), helping to maintain genomic stability. We have found a higher rate of D3S1611 MSI in older patients, suggesting that this marker may be affected by aging processes in the DNA repair machinery.