Development and Validation of a Stability Indicating RP-HPLC Method for the Determination of Two Sun Protection Factors (Koptrizon and Tinosorb S) in Topical Pharmaceutical Formulations Using Experimental Designs

Table 5

The range and levels of the variables in the 2³ full factorial design.

Std.	Run	Factor 1	Factor 2	Factor 3	Response 1	Response 2	Response 3
		A: flow rate	B: column temp	C: THF comp	RT of KPT	RT of TIN	Plate count of KPT
		(mL min^−1)	(°C)	(%)	(min)	(min)	(min)
0	2	1.5	50	38	2.8	4.3	3476
0	7	1.5	50	38	2.8	4.2	3346
7	1	1.3	55	43	2.2	3.2	2806
4	3	1.7	55	33	3.1	4.7	3200
2	4	1.7	45	33	3.9	6.0	4108
6	5	1.7	45	43	2.2	3.2	2767
8	6	1.7	55	43	1.8	2.6	2204
3	8	1.3	55	33	3.8	5.8	4114
5	9	1.3	45	43	2.8	4.1	3336
1	10	1.3	45	33	5.1	7.9	4804
0	2	1.5	50	38	2.8	4.3	3476