International Scholarly Research Notices

Research Article

Influence of Mikania laevigata Extract over the Genotoxicity Induced by Alkylating Agents

Table 2

Detection of DNA damage by comet assay (DF and DI) in blood cells of mice exposed to water, Mikania laevigata extract (MLE), and/or cyclophosphamide (CP) or methyl methanesulfonate (MMS) and sampled at 24 h (with and without pretreatment with MLE) or 48 h (with and without posttreatment with MLE).


Substances	Single doses (mg/Kg)	Schedule and comet assay parameters
Substances	Single doses (mg/Kg)	24 h	48 h	Pretreatment with MLE extract^a	Posttreatment with MLE extract^b

		Damage index
		DI ± SD	DI ± SD	DI ± SD	DI ± SD

Water ()	—	2.3 ± 1.5	8.3 ± 3.7^e	—	—
MLE ()	—	2.3 ± 1.1	7.3 ± 4.1^e	—	—
MMS ()	40.00	91 ± 3.6^c,d	64.7 ± 4.2^e	43.8 ± 5.1^e	43 ± 9.5^f
CP ()	25.00	45.8 ± 6.8^c	31.3 ± 3.6^e	18.3 ± 7.2^e	11 ± 3.0^f

		Damage frequency
		DF ± SD	DF ± SD	DF ± SD	DF ± SD

Water ()	—	2.0 ± 1.0	7.2 ± 3.9^e	—	—
MLE ()	—	2.3 ± 1.1	5.5 ± 2.4^e	—	—
MMS ()	40.00	73.5 ± 1.9^c,d	45.5 ± 5.7^e	31.7 ± 3.6^e	36.3 ± 10.7^f
CP ()	25.00	34.8 ± 4.6^c	22.3 ± 5.1^e	14.5 ± 6.9^e	10.2 ± 2.3^f

DI: damage index; DF: damage frequency; : number of individuals obtained from sum of independent experiments.
^aGroup sampled 24 h after treatment with an alkylating agent.
^bGroup sampled 48 h after treatment with an alkylating agent.
^cData significant in relation to water, EML in 24 h, < 0.01 (ANOVA, Tukey).
^dData significant in relation to group CP, < 0.01 (ANOVA, Tukey).
^eData significant in relation to 24 h at, < 0.001 (t-test).
^fData significant in relation to 48 h at, < 0.001 (t-test).