The Use of Response Surface Methodology as a Statistical Tool for Media Optimization in Lipase Production from the Dairy Effluent Isolate Fusarium solani

Table 1

Experimental design of Box-Behnken.

Std	Trial	Oil (%v/v)	(NH4)2SO4 (g/L)	CaCO3 (g/L)	Lipase (U)
15	1	3 (0)	5.5 (0)	0.55 (0)	43.68
10	2	3 (0)	10 (+1)	0.1 (−1)	46.81
2	3	5 (+1)	1 (−1)	0.55 (0)	56.11
1	4	1 (−1)	1 (−1)	0.55 (0)	44.03
12	5	3 (0)	10 (+1)	1 (+1)	42.08
7	6	1 (−1)	5.5 (0)	1 (+1)	56.18
11	7	3 (0)	1 (−1)	1 (+1)	39.49
8	8	5 (+1)	5.5 (0)	1 (+1)	70.32
3	9	1 (−1)	10 (+1)	0.55 (0)	45.2
9	10	3 (0)	1 (−1)	0.1 (−1)	43.18
16	11	3 (0)	5.5 (0)	0.55 (0)	43.68
13	12	3 (0)	5.5 (0)	0.55 (0)	43.68
6	13	5 (+1)	5.5 (0)	0.1 (−1)	73.34
14	14	3 (0)	5.5 (0)	0.55 (0)	43.68
5	15	1 (−1)	5.5 (0)	0.1 (−1)	59.09
4	16	5 (+1)	10 (+1)	0.55 (0)	61.52
17	17	3 (0)	5.5 (0)	0.55 (0)	43.68

(+1), (0) and (−1) are coded values.