Cell Transplantation and “Stem Cell Therapy” in the Treatment of Myopathies: Many Promises in Mice, Few Realities in Humans
Figure 4
Exogenous satellite cells are formed by the transplantation of human myoblasts in immunodeficient mice. The figure shows a single myofiber in a cross-section of a skeletal muscle of an immunodeficient mouse transplanted with myoblasts from an adult human donor 4 weeks before. Different elements are evidenced by fluorescent immunodetection in confocal microscopy. The peripheral nuclear labeling of human lamin A/C (green fluorescence) evidenced human nuclei in the periphery of the myofiber (visible by contrast enhancement). One of them (arrows) shows a typical intranuclear staining of Pax7, a marker of satellite cells (red fluorescence). Immunodetection of laminin (blue fluorescence) evidenced that this nucleus is in addition in the periphery of the myofiber and inside the basal lamina, that is, in the anatomical position of satellite cells (see Figure 5). Scale bar = 10 μm.
