Isolation and Characterization of Mycoplasma mycoides Subspecies capri from Milk of Natural Goat Mastitis Cases

Table 1

Details of primers used in study.

Primer	Specificity to species	Sequence	Target gene/region	Annealing; temperature	Reference
P4/P6	Mmc *; PCR	5′-ACTGAGCAATTCCTCTT-3′; 5′-TTAATAAGTCTCTATATGAAT-3′	CAP-21	46°C, 90 sec	[16]
P4/P5	Mmm LC*	5′-ACTGAGCAATTCCTCTT-3′; 5′-TTAAATAAGTTTGTATATGAAT-3′	CAP-21	54°C, 30 sec	[16]
Mag-F/Mag-R	M. ag*	5′-CCTTTTAGATTGGGATAGCGGATG-3′; 5′-CCGTCAAGGTAGCGTCATTTCCTAC-3′	16S  rRNA	60°C, 60 sec	[17]
MputF/MputR	M. put*	5′-AAATTGTTGAAAAATTAGCGCGAC-3′; 5′-CATATCATCAACTAGATTAATAGTAGCACC-3′	Arc B	52°C, 15 sec	[18]
MCCPL1-L/MCCPL1-R	Mcc *	5′-AGACCCAAATAAGCCATCCA-3′; 5′-CTTTCACCGCTTGTTGAATG-3′	LppA	51°C	[19]
P67BG7-L/P67BG7-R	Mbg7*	5′-GGTAATTCGAATAATGATCCT-3′; 5′-TAAGTTTATTGAATTAAAGCG-3′	P67 gene	46°C	[20]

∗Mmc: M. mycoides subsp. capri, Mmm LC: M. mycoides susp. mycoides large colony type, M. ag: M. agalactiae, Mcc: M. capricolum subsp. capricolum, Mbg7: M. bovine group 7.