Human Sprouty1 Suppresses Urokinase Receptor-Stimulated Cell Migration and Invasion

Figure 3

hSpry1 overexpression inhibits uPAR-stimulated tumor cell invasion. (a) MDA-MB-231 and (b) Saos-2 cells were transfected with pCDNA3.1 (light bars) or pCDNA3.1/hSpry1 (dark bars), placed in Transwell inserts, and were exposed to either 10% FCS, 1% FCS, or 100 ng/mL of EGF, uPA, or ATF. Cell invasion was assessed by counting (c) MDA-MB-231 and (d) Saos-2 cells on the membranes after 18 h. Bars are mean ± SEM (𝑛=5), and significance was measured using unpaired t-test (^∗𝑃<0.05; ^∗∗𝑃<0.01; ^∗∗∗𝑃<0.001). Data are representative of 3 independent experiments.

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