Changes in Water Sorption and Solubility of Dental Adhesive Systems after Cigarette Smoke

Vitória, Lívia Andrade; Aguiar, Thaiane Rodrigues; Santos, Poliana Ramos Braga; Cavalcanti, Andrea Nóbrega; Mathias, Paula

doi:https://doi.org/10.1155/2013/605847

International Scholarly Research Notices

On this page

Abstract Introduction Materials and Methods Results Discussion Conclusion Acknowledgments References Copyright Related Articles

Research Article | Open Access

Volume 2013 | Article ID 605847 | https://doi.org/10.1155/2013/605847

Changes in Water Sorption and Solubility of Dental Adhesive Systems after Cigarette Smoke

Lívia Andrade Vitória,¹Thaiane Rodrigues Aguiar,²Poliana Ramos Braga Santos,¹Andrea Nóbrega Cavalcanti,¹and Paula Mathias¹

Academic Editor: D. Wray, C. Lekic

Received16 Jun 2013

Accepted10 Jul 2013

Published28 Jul 2013

Abstract

Aim. To evaluate the effect of cigarette smoke on water sorption and solubility of four adhesive systems. Materials and Methods. Sixteen disks of each adhesive system were prepared (Adper Scotchbond Multipurpose Adhesive (SA); Adper Scotchbond Multipurpose Adhesive System (Adhesive + Primer) (SAP); Adper Single Bond Plus (SB); Adper Easy One (EO)). Specimens were desiccated until a constant mass was obtained and divided into two groups . One-half of the specimens were immersed in deionized water, while the other half were also immersed, but with daily exposure to tobacco smoke. After 21 days, disks were measured again and stored in desiccators until constant mass was achieved. Data were calculated according to ISO specifications and statistically analyzed. Results. The tobacco smoke only significantly affected the water sorption and solubility of EO. There were significant differences in both analyses among materials tested. The SB exhibited the highest water sorption, followed by EO, which demonstrated significantly higher solubility values than SB. The SA and SAP showed low water sorption and solubility, and there were no significant differences between the two. Conclusion. Regardless of smoke exposure, both simplified adhesive systems presented an inferior performance that could be related to the complex mixture of components in such versions.

1. Introduction

Many studies have shown that cigarette smoking is associated with deleterious health effects such as heart disease [1], chronic obstructive pulmonary disease, cancer [2], fertility problems [3], and periodontal disease [4]. In cosmetic restorative dentistry, some of the substances coming from tobacco can be absorbed by resin composite, dentin, and enamel surface [5] and lead to tooth/composite discoloration [6–10]. Moreover, bond strength reduction has been described due to the fact that cigarette particles may prevent effective contact between dentin and resin composite [11].

The mainstream smoke emitted from the mouth end is complex and composed by many physical and chemical processes [12]. In general, smoke formation is mostly produced by combustion and pyrolysis reactions and can be classified in two distinct phases. The first is called the vapour phase, consisting mainly of nitrogen, oxygen, carbon monoxide, carbon dioxide, acetaldehyde, methane, hydrogen cyanide, nitric acid, and acetone. The second category is the particulate phase. It consists of nicotine, water, and tobacco-specific nitrosamines ranging from 0.1 to 1.0 μm in diameter [12–17].

Beside many research reports on the staining effect of tobacco smoke on resin-based materials [6–9] and the positive correlation between tooth discoloration and duration-frequency of smoking habit [10], detailed information is not yet available concerning the effects of tobacco exposure on the physical and mechanical properties of dental restorative materials. Therefore, understanding the effects of cigarette smoke using a burning cigarette in vitro method can be helpful to predict the impact of cigarette smoking on the oral environment and on the long term of dental restorations.

In addition, water absorbed into the resin-based material might contribute to an accelerated degradation, promoting the deterioration of polymer resin [18–20]. The negative aspect of this mechanism includes softening of dental resin through plasticization, filler debonding, and residual monomer release of resin matrix that results in weight loss and characterizes the solubility behavior [19, 21]. Furthermore, water sorption and solubility can affect other properties (i.e., flexural and compressive strength and roughness [22, 23]) and have an important value in terms of the durability of restoration procedures.

Previous studies of water sorption and solubility have mainly been conducted in water [18, 19, 22, 24], artificial saliva [25], lactic acid [26], different pH beverages [23], ethanol, and chloroform [27]. To date, the ability of volatile compounds released during the cigarette combustion to affect the water sorption and solubility of resin-based materials is not yet described. Thus, the aim of the present study was to analyze whether cigarette smoke affects the water sorption and solubility of different adhesive systems. The null hypotheses tested were that (1) cigarette smoke has no effect on the water sorption and solubility of adhesive systems and, (2) none of the adhesive systems tested would have different performance.

2. Materials and Methods

2.1. Experimental Design

Four adhesive systems were investigated in this study: two conventional adhesive systems (Adper Scotchbond Multipurpose Adhesive and Adper Scotchbond Multipurpose System (Primer + Adhesive)); one two-step etch-and-rinse system (Adper Single Bond Plus) and one self-etching adhesive (Adper Easy One). Their composition specifications, manufacturer, and batch numbers are listed in Table 1.

2.2. Specimens Preparation

The sorption and solubility test was performed based on the ISO 4049 standard specification, except for the period of water immersion and cigarette smoke treatment. Sixteen disk-shaped specimens of each adhesive system were prepared ( mm in diameter × mm depth). The adhesive was directly applied to the metal mold, and a 60-second dwell time was used for solvent evaporation [28], followed by two seconds being gently blown with an oil/water-free compressed air at a distance of 10 cm. The specimens were light-cured for 15 seconds on the middle of the sample, and additional polymerization was applied at four equally peripheral points on the circumference lasting 10 seconds each, resulting in a total curing time of 55 seconds. The light-emitting diode curing unit (Radii-cal, SDI Dental Product, Bayswater Victoria, Australia) was used with a light power density of 1.200 mW/cm². The output irradiance was measured with a radiometer unit (Gnatus, Ribeirão Preto, SP, Brazil).

2.3. Water Sorption/Solubility and Smoke Exposure

Immediately after polymerization, all specimens were stored in a desiccator filled with anhydrous calcium sulphate (CaSO₄) at 37°C for 24 hours. Specimens were weighed using an electronic balance (AUD 220D, Shimadzu Corp., Nakagyo-ku, Kyoto, Japan) to an accuracy of ±0.1 mg. This procedure was repeated until a constant mass was obtained (), that is, until the mass loss of each specimen was not more than 0.1 mg within a period of 24 hours. The diameter and thickness of each specimen were measured using a digital electronic caliper (Mitutoyo Sul Americana Ltda, São Paulo, SP, Brazil), rounded to the nearest 0.01 mm. These measurements were taken in order to calculate the volume of each specimen in mm³.

Half of the specimens () were placed into polyethylene vials containing 5 mL of deionized water and individually stored for 21 days, while the other half was exposed to tobacco smoke and deionized water. For tobacco smoke-water treatment, specimens were daily exposed to tobacco smoke (10 cigarettes per 8 minutes, twice a day) [8, 9], washed and stored in deionized water after each cycle of smoke exposure for 21 days. The deionized water was renewed daily for all groups.

The cigarettes used presented an elevated content of tar (10 mg; Hollywood Original Blend, Souza Cruz SA, São Paulo, Brazil). The method used in this study was described in previous reports [8, 9, 29]. The tobacco smoke apparatus consisted of two chambers connected by orifices with cigarette filter papers, hermetically sealed. Lit cigarettes were placed in the first chamber that received external ventilation from an air pump, providing constant airflow. The specimens were placed in the second chamber where smoke-air was drawn through from the first chamber and had to overcome the tar filter barrier. In the second chamber, there was a special orifice to release the air stream.

After 21 days, specimens had excess water gently wiped off with absorbent paper and weighed (). Then, disks were conditioned again in the same manner as described before until a constant weight was achieved (). The values of water sorption (WS) and solubility (SL) were calculated as where is the initial dry constant mass (μg) prior to immersion in water; is the mass of the specimen (μg) after immersion in water or submitted to smoke treatment/water immersion for 21 days; is the mass of the reconditioned specimen (μg), and is the specimen volume in mm³.

2.4. Statistical Analysis

Water sorption and solubility were expressed in μg/mm³ and the data were analyzed by two-way ANOVA and Tukey tests. Statistical analysis was carried out in the SAS 9.1 statistical package (SAS Institute, Cary, NC, USA) with a 95% confidence level.

3. Results

The results of the experimental groups are presented in Table 2. A statistically significant interaction between adhesive system and cigarette smoke condition was observed for water sorption () and solubility (). The cigarette smoke significantly increased the water sorption and solubility of the self-etching adhesive system (Adper Easy One).

Regardless of the experimental condition, the adhesive Adper Single Bond Plus showed significantly higher water sorption values than all the other materials. On the other hand, the Adper Easy One exhibited higher solubility than Adper Single Bond Plus. The Adper Scotchbond Multipurpose Adhesive and Adper Scotchbond Multipurpose Systems (Adhesive + Primer) exhibited the lowest water sorption and solubility values, and there were no significant differences between them.

4. Discussion

Cigarette smoke is a dynamic mixture of at least 5600 chemicals and toxicants compounds. Some investigations have focused on the identification and quantifying of the particulate phase and volatile smoke components [12, 16, 17]. According to Bartalis et al. [17], the radicals detected in cigarette smoke are reactive and may promote oxidation; however, little is known about the effect of smoke cigarette on the physical and mechanical properties of resin-based materials. In this study, smoke exposure (10 cigarettes—8 minutes/twice a day/21 days) led to an increase of water sorption (12.5%) and solubility (7.9%) of Adper Easy One self-etching adhesive. Therefore, the first hypothesis was rejected since cigarette smoke significantly affected the water sorption and solubility of the self-etching adhesive system.

Most current adhesive systems are based on hydrophilic monomers for bonding to wet dentin substrate. The chemical composition of resin-based materials (i.e., hydrophilic and hydrophobic monomers, solvent content, and filler particles) is deemed to play crucial role in water sorption/solubility [18, 19, 21, 24, 30–32]. In the present study, the higher water sorption values were obtained for two-step etch-and-rinse Adper Single Bond Plus (268.5 and 275.1 μg/mm³), followed by self-etching Adper Easy One (216.5 and 243.6 μg/mm³) from two groups studied (control and cigarette smoke, resp.). Since changes in water sorption and solubility among adhesive systems were statistically observed, the second hypothesis was rejected.

In agreement with others studies, simplified adhesive systems have more acidity by increasing the concentration of ionic and/or acidic monomers [19, 21] and hence may result in more water uptake and solubility as showed in this investigation. Differences in amount of solvent and monomers constituents may lead to higher water sorption for Adper Single Bond Plus than for Adper Easy One.

On the other hand, conventional adhesive systems (Adper Scotchbond Multipurpose Adhesive and Primer/Adhesive) exhibited the lower water sorption, and there were no significant differences between them. The results indicated that hydrophobic resin coating (Adper Scotchbond Multipurpose Adhesive) has similar behavior when it was used alone or with additional hydrophilic primer (Adper Scotchbond Multipurpose Primer). Thus, these findings seem to indicate that additional hydrophobic resin coating can improve performance of dental adhesive.

In regard to solubility behavior, Adper Easy One demonstrated significantly higher values than Adper Single Bond Plus, followed by conventional systems. According to Ito et al. [18], higher water sorption value may be related to higher solubility. The high concentration of hydrophilic monomers in simplified adhesives can affect the vapor pressure of volatile components [30, 33]. Thus, after polymerization, nanopores may be present due to residual solvent evaporation and hence increase the water uptake. In addition, the simplified etch-and-rinse adhesive showed better ethanol solvent evaporation and monomer infiltration using 60-second dwell times [28]. Solvent evaporation was performed in this study; however, 60-second dwell time plus 2 seconds gently blown at a distance of 10 cm may not be sufficient to remove most of the water/ethanol for all tested adhesive systems. It can be speculated that solvent present in simplified systems may still remain in the adhesive and tends to promote more water sorption and solubility than in conventional systems.

The solubility results of Adper Scotchbond Multipurpose Adhesive and Primer/Adhesive did not show any significant differences. Two possible explanations for the negative solubility values are assumed. The first one was that the absorbed water during storage period may have not been completely eliminated, and consequently mass gain was obtained [27, 30, 32]. Secondly, the volatile smoke compounds may be linked to the adhesive systems specimens and led to increase the mass values.

It is well established that resin-based materials change color when exposed to cigarette smoke [7–9], and smoke staining agents were not removed after composite resin repolishing procedures [7]. Although the chemical mechanisms between cigarette staining and resin-based materials are not clear yet, cigarette-staining agents likely interacted with resin composite that is not only superficial. Thus, it is supposed that this interaction may be extended to adhesives systems due to the fact that adhesive systems have a greater amount of organic matrix than resin composite. In addition, high temperature exposure increases the rate of diffusion and the water sorption/solubility of dental adhesive, resulting in an irreversible degradation of the polymer network [32]. These results may be due to short-term effects of cigarette smoke and water storage used in this investigation or even to the fact that the temperatures reached by lit cigarettes were not high enough to provide matrix chemical degradation.

Although the adhesive layer does not stay fully exposed to cigarette smoke under clinical conditions, gaps may be present along the adhesive interface. Thus, in vitro study may possibly optimize this challenge, allowing for the analysis of the materials’ behavior in extreme conditions. In addition, more efforts are needed in this area to understand (1) the impact of tobacco products on the physical properties of resin-based materials and also (2) the chemical interaction between volatile smoke compounds and these materials, including some factors such as the presence and type of the paper cigarette filters, frequency, and intensity of habit.

5. Conclusion

The water sorption and solubility values were significantly different among the adhesives tested, except for conventional systems. The cigarette smoke only significantly affected the water sorption and solubility of the self-etching adhesive system. Thus, the cigarette smoke can increase the water sorption and solubility of adhesive systems, promoting a decline in their physical properties and consequently a reduction in the service life of dental resin-bonds.

Acknowledgments

This investigation was partially supported by CNPQ (PIBIC-UFBA), Brazil. The authors thank 3 M ESPE for the kind donation of materials for this investigation.

References

J. M. Bradley, J. B. Nguyen, A. C. Fournett, and J. D. Gardner, “Cigarette smoke exacerbates ventricular remodeling and dysfunction in the volume overloaded heart,” Microscopy and Microanalysis, vol. 18, no. 1, pp. 91–98, 2012.
View at: Publisher Site | Google Scholar
A. G. Schwartz, “Genetic epidemiology of cigarette smoke-induced lung disease,” Proceedings of the American Thoracic Society, vol. 9, no. 2, pp. 22–26, 2012.
View at: Google Scholar
C. Dechanet, T. Anahory, J. C. Mathieu Daude et al., “Effects of cigarette smoking on reproduction,” Human Reproduction Update, vol. 17, no. 1, pp. 76–95, 2011.
View at: Publisher Site | Google Scholar
U. Van der Velden, A. Varoufaki, J. W. Hutter et al., “Effect of smoking and periodontal treatment on the subgingival microflora: a retrospective study,” Journal of Clinical Periodontology, vol. 30, no. 7, pp. 603–610, 2003.
View at: Publisher Site | Google Scholar
C. Y. G. Takeuchi, A. M. Corrêa-Afonso, H. Pedrazzi, W. Dinelli, and R. G. Palma-Dibb, “Deposition of lead and cadmium released by cigarette smoke in dental structures and resin composite,” Microscopy Research and Technique, vol. 74, no. 3, pp. 287–291, 2011.
View at: Publisher Site | Google Scholar
J. Z. Bazzi, M. J. Bindo, R. N. Rached, R. F. Mazur, S. Vieira, and E. M. de Souza, “The effect of at-home bleaching and toothbrushing on removal of coffee and cigarette smoke stains and color stability of enamel,” Journal of the American Dental Association, vol. 143, no. 5, pp. e1–e7, 2012.
View at: Google Scholar
P. Mathias, T. A. Rossi, A. N. Cavalcanti, M. J. P. Lima, C. M. Fontes, and G. D. R. Nogueira-Filho, “Cigarette smoke combined with staining beverages decreases luminosity and increases pigmentation in composite resin restorations,” Compendium of Continuing Education in Dentistry, vol. 32, no. 2, pp. 66–70, 2011.
View at: Google Scholar
P. Mathias, L. Costa, L. O. Saraiva, T. A. Rossi, A. N. Cavalcanti, and G. da Rocha Nogueira-Filho, “Morphologic texture characterization allied to cigarette smoke increase pigmentation in composite resin restorations,” Journal of Esthetic and Restorative Dentistry, vol. 22, no. 4, pp. 252–259, 2010.
View at: Publisher Site | Google Scholar
P. Mathias, L. D. G. Silva, L. de Oliveira Saraiva et al., “Effect of surface sealant and repolishing procedures on the color of composite resin exposed to cigarette smoke,” General Dentistry, vol. 58, no. 4, pp. 331–335, 2010.
View at: Google Scholar
M. N. Alkhatib, R. D. Holt, and R. Bedi, “Smoking and tooth discolouration: findings from a national cross-sectional study,” BMC Public Health, vol. 5, article 27, 2005.
View at: Publisher Site | Google Scholar
J. S. Almeida e Silva, E. M. de Araujo Jr., and E. Araujo, “Cigarette smoke affects bonding to dentin,” General Dentistry, vol. 58, no. 4, pp. 326–330, 2010.
View at: Google Scholar
C. Liu, S. Feng, J. Van Heemst, and K. G. McAdam, “New insights into the formation of volatile compounds in mainstream cigarette smoke,” Analytical and Bioanalytical Chemistry, vol. 396, no. 5, pp. 1817–1830, 2010.
View at: Publisher Site | Google Scholar
M. F. Dube and C. R. Green, “Methods of collection of smoke for analytical purposes,” Recent Advances in Tobacco Science, vol. 8, pp. 42–102, 1982.
View at: Google Scholar
C. J. Smith and T. H. Fischer, “Particulate and vapor phase constituents of cigarette mainstream smoke and risk of myocardial infarction,” Atherosclerosis, vol. 158, no. 2, pp. 257–267, 2001.
View at: Publisher Site | Google Scholar
M. Borgerding and H. Klus, “Analysis of complex mixtures—cigarette smoke,” Experimental and Toxicologic Pathology, vol. 57, no. 1, pp. 43–73, 2005.
View at: Publisher Site | Google Scholar
J. Adamson, S. Hughes, D. Azzopardi, J. McAughey, and M. D. Gaça, “Real-time assessment of cigarette smoke particle deposition in vitro,” Chemistry Central Journal, vol. 6, article 98, 2012.
View at: Google Scholar
J. Bartalis, W. G. Chan, and J. B. Wooten, “A new look at radicals in cigarette smoke,” Analytical Chemistry, vol. 79, no. 13, pp. 5103–5106, 2007.
View at: Publisher Site | Google Scholar
S. Ito, T. Hoshino, M. Iijima, N. Tsukamoto, D. H. Pashley, and T. Saito, “Water sorption/solubility of self-etching dentin bonding agents,” Dental Materials, vol. 26, no. 7, pp. 617–626, 2010.
View at: Google Scholar
J. Malacarne, R. M. Carvalho, M. F. de Goes et al., “Water sorption/solubility of dental adhesive resins,” Dental Materials, vol. 22, no. 10, pp. 973–980, 2006.
View at: Publisher Site | Google Scholar
K. J. Söderholm, “Degradation of glass filler in experimental composites,” Journal of Dental Research, vol. 60, no. 11, pp. 1867–1875, 1981.
View at: Google Scholar
S. Ito, M. Hashimoto, B. Wadgaonkar et al., “Effects of resin hydrophilicity on water sorption and changes in modulus of elasticity,” Biomaterials, vol. 26, no. 33, pp. 6449–6459, 2005.
View at: Publisher Site | Google Scholar
A. Piwowarczyk and H.-C. Lauer, “Mechanical properties of luting cements after water storage,” Operative Dentistry, vol. 28, no. 5, pp. 535–542, 2003.
View at: Google Scholar
I. M. Hamouda, “Effects of various beverages on hardness, roughness, and solubility of esthetic restorative materials,” Journal of Esthetic and Restorative Dentistry, vol. 23, no. 5, pp. 315–322, 2011.
View at: Publisher Site | Google Scholar
M. Bail, J. Malacarne-Zanon, S. M. A. Silva et al., “Effect of air-drying on the solvent evaporation, degree of conversion and water sorption/solubility of dental adhesive models,” Journal of Materials Science. Materials in Medicine, vol. 23, no. 3, pp. 629–638, 2012.
View at: Publisher Site | Google Scholar
L. Musanje and B. W. Darvell, “Aspects of water sorption from the air, water and artificial saliva in resin composite restorative materials,” Dental Materials, vol. 19, no. 5, pp. 414–422, 2003.
View at: Publisher Site | Google Scholar
H. Y. Marghalani, “Sorption and solubility characteristics of self-adhesive resin cements,” Dental Materials, vol. 28, no. 10, pp. 187–198, 2012.
View at: Google Scholar
L. G. Lopes, A. D. V. Jardim Filho, J. B. de Souza, D. Rabelo, E. B. Franco, and G. C. de Freitas, “Influence of pulse-delay curing on sorption and solubility of a composite resin,” Journal of Applied Oral Science, vol. 17, no. 1, pp. 27–31, 2009.
View at: Publisher Site | Google Scholar
S. Argolo, D. C. Oliveira, C. M. Fontes, A. F. Lima, A. P. de Freitas, and A. N. Cavalcanti, “Effect of increased dwell times for solvent evaporation on the bond strength and degree of conversion of an ethanol-based adhesive system,” Acta Odontológica Latinoamericana, vol. 25, no. 1, pp. 109–114, 2012.
View at: Google Scholar
S. M. Le Mesurier, B. W. Stewart, and A. W. J. Lykke, “Injury to type-2 pneumocytes in rats exposed to cigarette smoke,” Environmental Research, vol. 24, no. 1, pp. 207–217, 1981.
View at: Google Scholar
J. Malacarne-Zanon, D. H. Pashley, K. A. Agee et al., “Effects of ethanol addition on the water sorption/solubility and percent conversion of comonomers in model dental adhesives,” Dental Materials, vol. 25, no. 10, pp. 1275–1284, 2009.
View at: Publisher Site | Google Scholar
I. Sideridou, V. Tserki, and G. Papanastasiou, “Study of water sorption, solubility and modulus of elasticity of light-cured dimethacrylate-based dental resins,” Biomaterials, vol. 24, no. 4, pp. 655–665, 2003.
View at: Publisher Site | Google Scholar
P. Dhanpal, C. K. Y. Yiu, N. M. King, F. R. Tay, and N. Hiraishi, “Effect of temperature on water sorption and solubility of dental adhesive resins,” Journal of Dentistry, vol. 37, no. 2, pp. 122–132, 2009.
View at: Publisher Site | Google Scholar
E. L. Pashley, Y. Zhang, P. E. Lockwood, F. A. Rueggeberg, and D. H. Pashley, “Effects of HEMA on water evaporation from water-HEMA mixtures,” Dental Materials, vol. 14, no. 1, pp. 6–10, 1998.
View at: Google Scholar

Copyright

Copyright © 2013 Lívia Andrade Vitória et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

PDF Download Citation

Download other formats

Order printed copies

Views

2759

Downloads

1137

Citations