Stability Indicating HPTLC Method for Analysis of Rifaximin in Pharmaceutical Formulations and an Application to Acidic Degradation Kinetic Study

Table 6

Degradation study of rifaximin in various conditions.

Exposure conditions	Degradation products (𝑅𝑓)	Recovery (%)^a
None (control sample)	Not detected	100
0.1 M HCl, refluxed (30 min)	0.44, 0.49	55.39
0.1 M NaOH, refluxed (30 min)	0.46, 0.51	81.94
3% Hydrogen peroxide, refluxed (30 min)	0.47	94.95
Wet heat, refluxed (30 min)	—	100
Sunlight (24 h)	0.67	89.72
UV light, 254 nm (24 h)	0.49	98.34

^a𝑛=3 replicates for concentration of 1000 ng/band.