Variable-Temperature Size Exclusion Chromatography for the Study of the Structural Changes in G-Quadruplex
Figure 3
Melting experiment of ckit21T18 monitored simultaneously by CD and molecular absorption spectroscopies. (a) Experimental CD spectra. (b) Experimental molecular absorption spectra. (c) Calculated distribution diagram. (d) Calculated pure CD and molecular absorption spectra. Concentration of DNA equal to 3.3 microM, pH 7.1, and 150 mM ionic strength.
