Variable-Temperature Size Exclusion Chromatography for the Study of the Structural Changes in G-Quadruplex

Figure 4

Calibration of chromatographic system. The oligonucleotides injected are 5′-T₂₁-3′ (MW: 6326.2 g·mol⁻¹), 5′-T₂₄-3′ (MW: 7238.7 g·mol⁻¹), 5′-ACC CCC TGC ATC TGC ATG CCC CCT CCC ACC CCC T-3′ (MW: 10,057.5 g·mol⁻¹), 5′-ACC CCC TGC ATC TTT TTG CCC CCT CCC ACC CCC T-3′ (MW: 10,076.5 g·mol⁻¹), and the Watson-Crick duplex formed by 5′-ACC CCC TGC ATC TGC ATG CCC CCT CCC ACC CCC T-3′ and 5′-AGG GGG TGG GAG GGG GCA TGC AGA TGC AGG GGG T-3′ (MW: 20,872.7 g·mol⁻¹). All of these sequences form lineal structures at pH 7.1 and 20°C.