Proteoglycan Expression in Normal Human Prostate Tissue and Prostate Cancer

Table 1

Primer sequences and PCR conditions.

Gene	Sequences	DNA fragment, bp	PCR conditions
			Annealing T, °C	Cycles
Gly	F 5′-GAGCTGCGGCGAGGTCCG-3′	648	55	33
	R 5′-CTGGTCTACTGTGCTCACTGCC-3′
Perl	F 5′-TCCCTGGACACAGATGGC-3′	314	55	35
	R 5′-ACCCATGCAGAAACAGGG-3′
Synd	F 5′-GCCCCCTGAAGATCAAGATGG C-3′	790	61	30
	R 5′-CCTCCTGTT TGGTGGGCTTCTG-3′
Agg	F 5′-CTT CTA CCG CCC CAC TGG CC-3′	400	63	33
	R 5′-GCCAGCCGGCGTCACACTG-3′
Vers	F 5′-GGCCAGCCCCCTGTTGTAGA-3′	308	63	33
	R 5′-AGGGATCAGCGCCTCGACTC-3′
NG2	F 5′-AGCCCTTTTGGGAGGCCCATG-3′	758	61	30
	R 5′-GAAGATGCCTGCCACGCTGC-3′
Brev	F 5′-GCCCTCACCATCCCTTGCCA-3′	476	61	30
	R 5′-TCCGACAGCCAGCCAGCATC-3′
Dec	F 5′-GGCCACTATCATCCTCCTTCTGC-3′	1031	59	25
	R 5′-ATGGCAGAGCGCACGTAGACAC-3′
Lum	F 5′-CTCTCTTCCTGGCATTGATTGGTGG-3′	720	61	30
	R 5′-GACAGATCCAGCTCAACCAGGG-3′
GAPDH	F 5′-GGGCGCCTGGTCACCAG-3′	350	59	22
	R 5′-AACATGGGGGCATCAGCAGAG-3′