Subcutaneous Administration of D-Luciferin is an Effective Alternative to Intraperitoneal Injection in Bioluminescence Imaging of Xenograft Tumors in Nude Mice

<table>BLI after SC versus IP injection at different anatomic sites. Nude mice bearing (a) U87-Luc bilateral subcutaneous thigh xenografts (5 mice), (b) GBM12-Luc intracranial brain xenografts (3 mice), (c) OSC19-Luc tongue xenografts (5 mice), or (d) A549-Luc pulmonary xenografts (2 mice) were imaged at 7 min after SC injection and 7 min after IP injection after an intervening washout period. Shown are representative images after SC (right mouse) or IP (left mouse) injection of D-luciferin. For each anatomic site, the graph shows the average BLS intensity (photon/sec/m<sup>2</sup>/1 min) for IP and SC <svg height="15.8" id="M12" style="vertical-align:-3.50803pt" version="1.1" viewbox="0 0 110.4875 15.8" width="110.4875" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink">
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</svg> after 3 distinct imaging sessions. No statistically significant differences were noted.</table>

International Scholarly Research Notices

fig3

Figure 3

Figure 3: Subcutaneous Administration of D-Luciferin is an Effective Alternative to Intraperitoneal Injection in Bioluminescence Imaging of Xenograft Tumors in Nude Mice 

Figure 3 | Subcutaneous Administration of D-Luciferin is an Effective Alternative to Intraperitoneal Injection in Bioluminescence Imaging of Xenograft Tumors in Nude Mice 