International Scholarly Research Notices

Research Article

Identification of Appropriate Reference Genes for qRT-PCR Analysis of Heat-Stressed Mammary Epithelial Cells in Riverine Buffaloes (Bubalus bubalis)

Table 5

Parameters based cycle point (CP) values for the 10 most stable housekeeping genes.


	EEF1A1	RPL4	RPS23	GTP	UXT	RPS9	RPS15A	HMBS	B2M	HPRT1

	9	9	9	9	9	9	9	9	9	9
GM [CP]	23.64	22.02	19.81	24.94	25.90	19.15	19.15	25.81	22.07	22.57
AM [CP]	23.64	22.02	19.81	24.94	25.90	19.54	19.15	25.81	22.07	22.57
min [CP]	23.31	21.79	19.42	24.54	25.70	10.27	18.80	25.31	21.86	22.14
max [CP]	23.91	22.30	20.02	25.27	26.30	20.87	19.43	26.06	22.37	23.00
SD [±CP]	0.13	0.11	0.15	0.16	0.12	2.06	0.13	0.18	0.11	0.20
CV [%CP]	0.57	0.52	0.76	0.64	0.47	10.54	0.67	0.71	0.49	0.89
min [x-fold]	−1.26	−1.18	−1.31	−1.32	−1.15	−470.28	−1.28	−1.42	−1.16	−1.34
max [x-fold]	1.21	1.21	1.16	1.25	1.31	3.30	1.21	1.19	1.23	1.35
SD [±x-fold]	1.10	1.08	1.11	1.12	1.09	4.17	1.09	1.13	1.08	1.15

: number of samples; GM [CP]: geometric mean of cycling point; AM [CP]: arithmetic mean of CP; min [CP] and max [CP]: extreme values of CP; SD [±CP]: standard deviation of the CP; CV [%CP]: coefficient of variation expressed as a percentage on the CP values; min [x-fold] and max [x-fold]: extreme values of expression levels expressed as absolute x-fold over or under coefficient; SD [±x-fold]: standard deviation of the absolute regulation coefficients.