Identification of Appropriate Reference Genes for qRT-PCR Analysis of Heat-Stressed Mammary Epithelial Cells in Riverine Buffaloes (Bubalus bubalis)

Table 6

Repeated pairwise correlation analysis among genes and with BestKeeper index (BI).

Genes	versus	EEF1A1	RPL4	RPS23	GTP	UXT	RPS9	RPS15A	HMBS	B2M	HPRT1
RPL4	R	0.893	—	—	—	—	—	—	—	—	—
	P value	0.001	—	—	—	—	—	—	—	—	—
RPS23	R	0.841	0.800	—	—	—	—	—	—	—	—
	P value	0.005	0.010	—	—	—	—	—	—	—	—
GTP	R	0.790	0.592	0.718	—	—	—	—	—	—	—
	P value	0.011	0.094	0.029	—	—	—	—	—	—	—
UXT	R	0.776	0.426	0.519	0.793	—	—	—	—	—	—
	P value	0.014	0.251	0.151	0.011	—	—	—	—	—	—
RPS9	R	0.712	0.608	0.766	0.351	0.464	—	—	—	—	—
	P value	0.032	0.082	0.016	0.355	0.207	—	—	—	—	—
RPS15A	R	0.507	0.250	0.592	0.788	0.634	0.363	—	—	—	—
	P value	0.163	0.518	0.094	0.012	0.067	0.337	—	—	—	—
HMBS	R	0.658	0.630	0.775	0.791	0.413	0.430	0.812	—	—	—
	P value	0.054	0.069	0.014	0.011	0.269	0.248	0.008	—	—	—
B2M	R	0.206	0.056	0.072	0.179	0.407	−0.042	0.384	0.168	—	—
	P value	0.593	0.885	0.855	0.646	0.277	0.915	0.308	0.666	—	—
HPRT1	R	0.382	0.305	0.273	0.639	0.368	0.146	0.676	0.743	−0.013	—
	P value	0.312	0.423	0.478	0.064	0.329	0.708	0.045	0.022	0.977	—
BestKeeper Index	R	0.804	0.679	0.840	0.503	0.565	0.983	0.497	0.567	0.035	0.273
	P value	0.009	0.044	0.005	0.167	0.113	0.001	0.172	0.112	0.931	0.478
	𝑟^2	0.646	0.461	0.706	0.253	0.319	0.966	0.247	0.321	0.001	0.075

𝑅: Pearson correlation coefficient; 𝑟²: coefficient of determination.