Research Article

Macromolecular Crowding Enhances Catalytic Efficiency and Stability of α-Amylase

Table 1

Reversibility of cosolvent’s induced enhancement of α-amylase activity. The enzyme activity was measured after treatment with cosolvents and removal of cosolvents from the protein solution.

Enzyme activity (×1000 unit)
CosolventsWithout any treatmentAfter treatment with cosolvents (40%, w/v)After removal of cosolvents

Buffer only2.6 ± 0.15
Glycerol3.6 ± 0.12.6 ± 0.25
Sorbitol3.7 ± 0.32.8 ± 0.15
Sucrose3.8 ± 0.252.4 ± 0.1
Trehalose4.1 ± 0.32.7 ± 0.2