Quality by Design Approach for the Development and Validation of Glipizide, an Antidiabetic Drug, by RP-UPLC with Application to Formulated Forms and Urine

Table 2

Final optimization of pH with Zorbax Extend C-18 (50×4.6) mm, 1.8 µm column.

S. no.	Trails taken	Observations	Remarks
1	ACN : buffer (pH 3.5) (40 : 60% v/v)	Peaks found symmetrical	Satisfactory
2	ACN : buffer (pH 4) (40 : 60% v/v)	Peak eluted before 1 min with less theoretical plates	Not satisfactory
3	ACN : buffer (pH 3) (40 : 60% v/v)	Broad peak	Not satisfactory
4	ACN : buffer (pH 5) (40 : 60% v/v)	Broad peak and late elution	Not satisfactory