Genotoxicological Evaluation of NUTRALYS Pea Protein Isolate

Table 2

Summary of average histidine revertants and induction ratios.

Metabolic activation	Test concentrations; (𝜇g/plate)	TA1535		TA1537		TA98		TA100		TA102
		AHR ± SD	Induction ratio	AHR ± SD	Induction ratio	AHR ± SD	Induction ratio	AHR ± SD	Induction ratio	AHR ± SD	Induction ratio
Without S9	^#Solvent control	37.33±2.08	—	9.00±4.58	—	37.00±4.36	—	200.00±7.81	—	352.00±11.27	—
	312.5	39.67±7.02	1.06	10.33±2.89	1.15	38.67±7.09	1.05	214.00±6.24	1.07	373.67±15.63	1.06
	625	38.33±7.37	1.03	7.33±4.04	0.81	46.67±9.45	1.26	203.00±12.17	1.02	349.33±18.82	0.99
	1250	31.00±6.08	0.83	11.33±2.89	1.26	36.67±9.24	0.99	214.67±4.04	1.07	324.33±9.02	0.92
	2500	34.33±4.62	0.92	12.00±3.00	1.33	39.33±7.77	1.06	209.33±9.45	1.05	240.33±5.51	0.68
	5000	24.33±4.04	0.65	8.00±3.61	0.89	29.67±6.11	0.80	193.67±13.05	0.97	242.00±6.00	0.69
	Positive Controls	418.33±21.22	—	121.00±30.51	—	311.33±2.89	—	1037.00±53.36	—	1639.67±44.64	—
With S9	^#Solvent control	37.33±7.23	—	7.33±2.08	—	36.67±7.23	—	187.33±8.02	—	386.67±20.50	—
	312.5	31.33±4.16	0.84	12.33±3.06	1.68	29.00±4.58	0.79	182.33±12.50	0.97	381.67±16.26	0.99
	625	28.33±1.53	0.76	11.67±5.03	1.59	26.67±5.03	0.73	209.67±2.31	1.12	383.33±8.62	0.99
	1250	38.33±2.08	1.03	9.67±3.06	1.32	36.33±10.07	0.99	191.00±12.29	1.02	359.00±3.46	0.93
	2500	25.33±4.16	0.68	7.00±1.73	0.95	48.33±6.66	1.32	145.33±15.89	0.78	377.33±11.59	0.98
	5000	31.67±10.26	0.85	7.67±0.58	1.05	32.00±4.00	0.87	136.33±8.62	0.73	365.33±10.41	0.94
	Benzo(a)pyrene (10 µg /plate)	517.67±64.63	—	76.67±5.51	—	584.00±36.37	—	1516.67±13.58	—	1397.33±421.17	—

AHR: Average histidine revertants, S.D.: standard deviation; induction ratio: mean number of revertants in treated/mean number of revertants in the control; ^#sterile millipore water 100 𝜇L/plate.