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Volume 2013 (2013), Article ID 824320, 8 pages
Gastroesophageal Reflux in Critically Ill Children: A Review
1Pediatric Intensive Care Department and Gastroenterology Section, Gregorio Marañón University General Hospital, 28009 Madrid, Spain
2Servicio de Cuidados Intensivos Pediátricos, Instituto de Investigación del Hospital Gregorio Marañón, Hospital General Universitario Gregorio Marañón, Dr. Castelo 47, 28009 Madrid, Spain
Received 21 December 2012; Accepted 10 January 2013
Academic Editors: J.-P. Buts, U. Klinge, I. Takeyoshi, and W. Vogel
Copyright © 2013 Maria José Solana García et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Gastroesophageal reflux (GER) is very common in children due to immaturity of the antireflux barrier. In critically ill patients there is also a high incidence due to a partial or complete loss of pressure of the lower esophageal sphincter though other factors, such as the use of nasogastric tubes, treatment with adrenergic agonists, bronchodilators, or opiates and mechanical ventilation, can further increase the risk of GER. Vomiting and regurgitation are the most common manifestations in infants and are considered pathological when they have repercussions on the nutritional status. In critically ill children, damage to the esophageal mucosa predisposes to digestive tract hemorrhage and nosocomial pneumonia secondary to repeated microaspiration. GER is mainly alkaline in children, as is also the case in critically ill pediatric patients. pH-metry combined with multichannel intraluminal impedance is therefore the technique of choice for diagnosis. The proton pump inhibitors are the drugs of choice for the treatment of GER because they have a greater effect, longer duration of action, and a good safety profile.
Gastroesophageal reflux (GER) occurs when gastric contents pass through the lower esophageal sphincter (LES) into the esophagus . Under normal conditions, reflux is prevented by correct function of the gastroesophageal junction, also known as the antireflux barrier.
GER is very common in children due to immaturity of the antireflux barrier. Clinical manifestations usually begin at 2 to 3 months of age  and are characterized by the regurgitation of milk, mostly in the postprandial period; however, the child’s growth and development are not affected .
The frequency of GER is higher in infants than in older children and adults, with prevalences of up to 85% . The male-to-female ratio is from 1.6 to 1. The higher prevalence is due to immaturity of the esophagus and stomach in infants and because most of the diet is ingested in liquid form .
Other risk groups include children with cerebral palsy, children requiring surgery to correct esophageal atresia, and patients with hiatus hernia . The administration of certain drugs that can relax the LES will also predispose to GER. These drugs include the anticholinergics, calcium-channel blockers, benzodiazepines, and dopamine . Additional risk factors that have been identified in adults are alcohol consumption, smoking, connective tissue diseases (particularly scleroderma) , and chronic obstructive pulmonary disease .
The antireflux barrier is formed by the lower esophageal sphincter (LES) and the diaphragmatic crural sling, which open during swallowing to permit the passage of the food bolus . Opening of the gastroesophageal junction depends on 3 factors: relaxation of the LES, inhibition of the diaphragmatic crural sling, and shortening of the esophagus [8, 9]. A fourth element, the positive pressure gradient present between the stomach and the gastroesophageal junction, also plays an important role .
The muscularis propria of the esophagus is formed of a circular muscle layer that generates pressure waves that transport food bolus and a longitudinal muscle layer that acts to shorten the esophagus. Synchrony between the 2 muscle layers produces effective peristalsis, which has a major influence on the pathophysiology of GER, as it avoids the harmful effects of acid reflux on the mucosa and prevents the appearance of complications such as esophagitis and stenosis.
There are 3 basic mechanisms that can lead to GER:(i)transient relaxation of the LES(ii)a transient increase in abdominal pressure that momentarily exceeds the competence of the sphincter(iii)low basal LES tone.
The most common cause of GER is transitory relaxation of the LES  although there are other factors that can also favor reflux, such as the placement of nasogastric tubes, slow gastric emptying [11, 12], neuronal and/or muscle dysfunction , and drug- or hormone-induced dysmotility .
Transitory episodes of relaxation of the LES can not only occur in children in association with swallowing, but can also develop when the stomach is distended by air or fluid. It would appear that a vagal mechanism (neither cholinergic nor adrenergic) is involved in LES relaxation, and nitric oxide may also be implicated .
During the initial weeks of life, it is already possible to detect the basal tone of the LES, which would indicate that GER occurs due to a transitory but repetitive loss of pressure caused by inappropriate relaxation of the LES rather than inadequate basal LES pressure .
It is important to take into account the influence of position on GER. A study that investigated the effect of position on GER in 10 healthy preterm infants with a gestational age of 35 to 37 weeks demonstrated that the right lateral position was associated with more episodes of reflux then the left lateral position even though gastric emptying was faster in the right lateral position . Additionally, the short length of the sphincter at this age and the lower efficacy of peristalsis, which leads to poor clearance of the refluxed material, mean that the incidence of reflux is higher.
GER in childhood usually resolves spontaneously between 12 and 18 months of age due to growth of the esophagus, an increase in LES tone, a solid diet, and less time spent in the supine position [16, 17].
4. Clinical Manifestations of Gastroesophageal Reflux in Children
In some series, the prevalence of symptoms of GER can be as high as 60% .
In adults, the most common symptoms of GER are heartburn and regurgitation; dysphagia, odynophagia, and chest pain are less frequent. Erosive esophagitis is present in 30% of patients with GER; the main cause is excessive and persistent presence of acid in the lower part of the esophagus. The maintenance of a gastric pH above 4 is therefore the most important strategy for controlling this disease.
The most common clinical manifestations in children are the following.
4.1. Digestive Tract Manifestations 
They are as follows(i)vomiting and regurgitation: these are the most common manifestations in infants and are considered pathological when they have repercussions on the infant’s nutritional status(ii)irritability and food rejection(iii)heartburn, dysphagia, and retrosternal pain, mainly in older children(iv)erosive esophagitis, Barrett’s esophagus, and esophageal stenosis, which are uncommon in children [14, 19].
4.2. Respiratory Tract Manifestations 
They are as follows(i)asthma and chronic cough(ii)laryngitis and stridor(iii)aspiration pneumonia(iv)apnea.
It is essential to take an adequate history that details the nature and frequency of vomiting .
5.1. Imaging Studies with Contrast
5.2. Gamma Scan
Endoscopy can be used to evaluate the state of the esophagus and degree of esophagitis as well as for identification or exclusion of other causes of esophagitis. In addition, the technique is useful for monitoring the clinical course of Barrett’s esophagus [2, 20]. Endoscopy is only indicated if there is a suspicion of complications of GER.
Until recently, monitoring the pH of the esophagus was considered to be the reference technique for the diagnosis of GER in children. This method detects acid reflux . An episode of acid reflux is defined as a fall in the esophageal pH to below 4 for at least 5 seconds [18, 22].
Some authors consider the presence of more than 9 episodes of acid GER per day to be pathological in children . Although the diagnostic criteria for GER in children vary, the most widely used at the present time for the diagnosis of this condition are the ones published by Boix-Ochoa  and by Vandeplas , which differ only slightly. The Boix-Ochoa scale integrates the mean duration of the episodes of GER, the clearance time, and the total time of GER to produce a score that is considered to be pathological at values over 6.6.
The most significant limitation of pH-metry is its poor capacity to detect episodes of alkaline reflux, which are common in infants and in patients on treatment with proton pump inhibitors. In addition, it cannot determine the characteristics of the refluxate (liquid, mixed, gas), the height reached by the refluxate in the esophagus, or its clearance .
5.5. Multichannel Intraluminal Impedance
Multichannel intraluminal impedance (MII) is a new technique that, in combination with pH-metry, increases the sensitivity and specificity of the detection of GER as it detects both episodes of acid and of alkaline reflux [18, 26, 27].
MII is based on the insertion of a nasogastric catheter with 6 esophageal electrodes and 1 or 2 gastric electrodes. The device provides a continuous recording of the changes in conductivity that occur in the esophagus due to the passage of food or air or due to GER .
Impedance is determined by the resistance to the passage of an electrical current between electrodes situated on the catheter inserted into the esophagus. When the esophagus is empty, the impedance is high at its walls that are in contact . A fall in the impedance of greater than 50% with respect to the baseline indicates the passage of a food bolus or other substances .
MII classifies the episodes of GER as acid if the refluxate causes the pH in the esophagus to fall below 4, alkaline if the pH rises above 7, and weakly acid if the esophageal pH falls by at least 1 unit but does not fall below 4 [28, 29].
In addition to detecting the content of the bolus, MII can identify its composition, as the impedance of the esophagus falls with liquid and mixed reflux but rises with gas reflux [26, 30]. Furthermore, MII can detect the direction and location of episodes of reflux independently of their pH.
Infants have a higher frequency of alkaline reflux due, in part, to their milk-based diet . This finding explains why the majority of episodes of GER in infants are not detected by pH-metry but can be detected when this technique is combined with MII .
The main limitation of MII is that there are no established reference values for children that enable a diagnosis of reflux to be made using this method, and we therefore have to resort to normalized indices for adults [33, 34].
6. Treatment of GER
The objectives of the treatment of GER are to relieve symptoms, cure esophagitis if it is present, and treat or prevent complications . Strategies to achieve these objectives include lifestyle changes, pharmacological therapy, and surgery.
6.1. General Measures
Recommendations on dietary and lifestyle changes depend on the age of the patient.
In infants, dietary modifications include thickening the milk and the introduction of solid foods although this measure only appears to reduce the symptoms and does not affect the number of episodes of reflux . Wenzl and cols.  showed antireflux formulas to be useful in reducing the number and severity of episodes of GER, particularly in cases of nonacid reflux.
The prone position is associated with a lower incidence of GER , but this is not recommended in neonates or infants due to its association with sudden infant death syndrome—the supine position is preferred in this age group . The use of the prone position should only be indicated in highly selected cases .
Elevation of the head of the bed to 30° does not appear to be an effective measure. A study performed by Bagucka and cols.  in 10 infants with continuous monitoring of the esophageal pH compared the horizontal position with an inclination of 30°. The authors analyzed the mean number of episodes of GER in each position and found a statistically significant difference in favor of the horizontal position. Recommendations in older children and adolescents are the same as those for adults: elimination from the diet of substances that relax the LES, such as caffeine, chocolate, alcohol, tobacco, and spices, and the avoidance of overweight .
6.2. Pharmacological Treatment
These agents are useful in patients with moderate symptoms .(i)Cisapride is a serotoninergic agent that increases acetylcholine release in the gastrointestinal tract. It acts on the LES and stomach to improve contractility and gastric peristalsis. In this way it improves symptoms and reduces esophageal and respiratory complications . However, at the present time cisapride is not indicated because it is associated with prolongation of the QT interval, arrhythmias, and sudden death.(ii)Domperidone is a dopaminergic receptor antagonist that reduces the duration of postprandial reflux. It is metabolized by cytochrome P450, and its plasma levels can therefore be affected by other substances that act on this enzyme. Its most undesirable adverse effect is the onset of extrapyramidal symptoms.(iii)Metoclopramide is an antidopaminergic agent with serotoninergic and cholinergic effects. It increases LES tone, improves esophageal peristalsis, and accelerates gastric emptying. The adverse effects of metoclopramide include extrapyramidal symptoms and tardive dyskinesia .(iv)Erythromycin, in addition to being an antibiotic, has a prokinetic effect through the direct activation of the motilin receptors . This drug has been shown to be effective for the treatment of dysmotility in premature infants and for diabetic and postoperative gastroparesis . Erythromycin has been shown to be useful in GER as it increases LES tone both during meals and in the postprandial period.
6.2.2. Inhibitors of Gastric Secretion
The H2-receptor antagonists bind to the histamine receptors of the parietal cells, producing competitive inhibition. This reduces acid secretion both in the basal situation and after stimulation by food, caffeine, insulin, or pentagastrin. The H2 antagonists also indirectly reduce pepsin secretion, and they have a cytoprotective effect on the gastric mucosa, favoring healing .
Proton Pump Inhibitors
The proton pump inhibitors (PPIs) are the drugs of choice for the treatment of gastroesophageal reflux and gastroduodenal ulcer [42–44]. They act by binding selectively and irreversibly to the proton exchange ATPase (H/K ATPase), forming disulphide bridges with the cystine residues of the subunit of the ATPase .
The advantages of the PPIs over the H2-receptor antagonists include a longer duration of action, more potent inhibition of acid secretion in the basal situation and after parietal cell stimulation, no induction of tolerance, and a better safety profile .
Five PPIs are available: omeprazole, lansoprazole, pantoprazole, rabeprazole, and esomeprazole. The differences in their molecular structure give rise to variations in their pharmacokinetic characteristics .
The PPIs have been shown to be more effective than the H2-receptor antagonists for symptom relief and for the treatment of erosive esophagitis in adults  and children ; this is due to their greater inhibition of acid secretion and their longer duration of action [46, 50]. These 2 groups of drugs were compared in a meta-analysis, which showed that the rates of symptom relief and of healing after 8 weeks of treatment were 77% and 85%, respectively, with the PPIs and 48% and 52%, respectively, with the H2-receptor antagonists. In addition, the symptoms of heartburn disappeared more rapidly in patients treated with PPIs .
Few studies have analyzed the efficacy of the H2-receptor antagonists and the PPIs in GER in children. One study performed in children with peptic esophagitis found that 70% of patients responded to treatment with ranitidine ; of the 30% that did not respond, 87% did respond to omeprazole. In another multicentre study of children with chronic esophagitis, omeprazole treatment resolved the esophagitis in 82% of patients and led to an improvement in symptoms (heartburn, epigastric pain, irritability, dysphagia, odynophagia, cough, wheeze, and vomiting) in 93% of cases .
There is only 1 study that has compared the efficacy of the H2-receptor antagonists with that of the PPIs for the treatment of GER in children. In 32 patients, the efficacy of high doses of ranitidine (20 mg/kg/d) was equal to that of normal doses of omeprazole (40 mg/d/1.73 m2) .
6.2.3. New Treatments
Agents That Reduce LES Relaxation
Gastric distension stimulates afferent vagal fibers that run from the stomach to the solitary tract nucleus (STN) and dorsal motor nucleus, producing a rapid relaxation of the LES . Glutamate and -aminobutyric acid (GABA) are the neurotransmitters of the neurons in the STN, and the inhibition of the afferent system is mediated by the GABAB receptors . Baclofen is a GABAB agonist that has been shown to be useful for the treatment of GER in children [55, 56].
Competitive ATPase Potassium-Channel Blockers
Soraprazan (BY359), revaprazan (YH1885), AZD0865, and CS-526 block acid secretion through a competitive and reversible inhibition of the potassium channels of the H+/K+ ATPase . At the present time there is little experience with these drugs.
Gastrin Inhibitor Drugs
Gastrin stimulates acid secretion through 3 mechanisms: direct stimulation of the parietal cell, increased histamine release from the enterochromaffin cells, and somatostatin release . Blockade of its receptors (the cholecystokinin 2 receptors) will therefore reduce acid secretion.
Some of the research in this field is aimed at developing an antigastrin vaccine that will stimulate the production of antibodies that neutralize this hormone .
6.2.4. Surgical Treatment
Surgery is indicated in children with severe respiratory problems such as aspiration, apnea, or laryngospasm, in patients with a poor pharmacological response due to motor disturbances of the esophagus that provoke continuous aspirations, children who do not tolerate the medication , and GER associated with esophagitis, malnutrition, persistent vomiting, or hiatus hernia .
In children with neurological disabilities, the indications for performing an antireflux procedure are the following: presence of episodes of apnea, bradycardia, recurrent pneumonia or apparent life threaetning events, Barrett’s esophagus, and the presence of a gastrostomy .
There are a number of surgical techniques: the Nissen total fundoplication, the anterior partial fundoplication (Thal fundoplication), and the posterior partial fundoplication (Toupet fundoplication) . Laparoscopic surgery is preferred [61, 62]. Fundoplication relieves symptoms in 57% to 92% of patients with a mortality that varies between 0% and 5% . The most common complication is dysphagia  although other postoperative complications can occur, such as dumping syndrome, dysphagia, vagal nerve paralysis, hemorrhage, infection, and adhesions .
7. Gastroesophageal Reflux in the Critically Ill Pediatric Patient
7.1. Incidence and Relevance
There is only 1 study that has analyzed the incidence of GER in critically ill children. Abdel-Gawad et al.  studied the incidence of GER using pH-metry combined with MII in 24 children on mechanical ventilation. They detected at least 1 episode of GER in 91.6% of patients. On dividing the patients into 2 groups, they found that 100% of patients that developed mechanical ventilation-related pneumonia presented GER compared with 75% of those that did not develop this type of pneumonia.
In our intensive care unit, using pH-metry combined with MII, we detected at least 1 episode of GER in 83% of critically ill children on mechanical ventilation and 36% satisfied the criteria for GER disease.
7.2. Characteristics of the Reflux in the Critically Ill Child
In contrast to adults, the episodes of GER in critically ill children are mainly alkaline . This could be due to prophylactic treatment with omeprazole or H2-receptor antagonists for digestive tract hemorrhage or to the presence of duodenogastric reflux ; however, these factors are also present in critically ill adult patients. This situation explains why impedance has a greater diagnostic sensitivity than pH-metry for the diagnosis of GER in the critically ill child .
The ability to clear the refluxate is greater in critically ill children than in adults. In our experience, esophageal clearance was more rapid in children than in adults (30.7 seconds versus 143 seconds) and the total time that the esophageal pH was below 4 was significantly lower (0.96% versus 39.4%).
In critically ill patients, GER mainly occurs due to a reduction or loss of pressure in the LES although it can also be triggered by cough and exertion . Other factors that increase the risk of GER are the supine position, the use of large nasogastric tubes , adrenergic agonists, bronchodilators, and opiates ; the risk of GER is also increased by mechanical ventilation associated with an inhibition of peristalsis and visceral hypoperfusion secondary to the use of PEEP , clinical severity , shock, sepsis , and cranial trauma .
In critically ill children we found no relationship between GER and the dose of inotropes or sedatives or the use of mechanical ventilation. Patients receiving muscle relaxants have less GER than other patients; this may be because of the relaxation of the gastric muscles caused by these drugs.
pH-metry and, particularly, MII are the methods of choice for the diagnosis of GER in the critically ill patient although there are no comparative data for the 2 techniques in adults.
In our experience, MII is able to detect a larger number of episodes of reflux per patient than pH-metry, probably because most of the episodes of reflux in children are alkaline and cannot therefore be recorded by pH-metry. However, although impedance detects a larger number of episodes of reflux overall, it has a lower ability than pH-metry to detect episodes of acid reflux. These results are very similar to those found in studies performed in noncritical children with GER, in whom approximately 70% of the episodes detected by pH-metry alone were not associated with retrograde movement .
7.5. Complications of GER in the Critically Ill Patient
In adults, GER increases the risk of digestive tract hemorrhage due to damage to the esophageal mucosa and increases the incidence of pulmonary microaspirations and nosocomial pneumonia; these alterations will affect morbidity and mortality, length of hospital stay, and economic cost [64, 71].
Abdel-Gawad et al.  found that mortality was higher among children with acid and mixed reflux than amongst those with alkaline reflux. Also, the duration of acid reflux, the number of episodes of acid reflux, the number of episodes of prolonged acid reflux (>5 minutes), the duration of the longest episode of acid reflux, and the acid-reflux index were higher in patients that died than in the survivors. In contrast, the parameters of alkaline reflux did not differ between children that died and survivors. These differences were not detected in our study, but the mortality was much lower (5.5%) than in the study by Abdel-Gawad et al. (58.3%) .
7.6. GER Prophylaxis and Treatment in the Critically Ill Patient
Elevation of the head of the bed to 45° and the use of small-diameter nasogastric tubes are measures that can reduce the incidence of GER in critically ill patients . The use of transpyloric tubes also helps to reduce GER because there is a smaller gastric residue and thus less gastric distension [72, 73].
The administration of prokinetic drugs can be useful to improve gastric hypomotility. Of these, erythromycin and metoclopramide are the most widely used in critically ill patients [73, 74]. The H2-receptor antagonists and proton pump inhibitors are useful drugs for the treatment of GER as they inhibit gastric acid secretion, increase the pressure of the LES, and improve esophageal acid clearance . Inhibitors of acid secretion, such as omeprazole, do not reduce the number of episodes of GER, but cause the episodes to be nonacidic and, therefore, less harmful to the esophageal mucosa .
GER is common in healthy infants and in critically ill children. pH-metry combined with MII is the technique of choice for the diagnosis of GER in children, including those with critical illness, because GER is typically alkaline in these patients. The proton pump inhibitors are the treatment of choice for the prophylaxis and treatment of GER.
- M. Pacilli, M. M. Chowdhury, and A. Pierro, “The surgical treatment of gastro-esophageal reflux in neonates and infants,” Seminars in Pediatric Surgery, vol. 14, no. 1, pp. 34–41, 2005.
- X. L. Liu and K. K. Y. Wong, “Gastroesophageal reflux disease in children,” Hong Kong Medical Journal, vol. 18, no. 5, pp. 421–428, 2012.
- S. J. Newell, I. W. Booth, M. E. I. Morgan, G. M. Durbin, and A. S. McNeish, “Gastro-oesophageal reflux in preterm infants,” Archives of Disease in Childhood, vol. 64, no. 6, pp. 780–786, 1989.
- J. P. Cezard, “Managing gastro-oesophageal reflux disease in children,” Digestion, vol. 69, no. 1, pp. 3–8, 2004.
- S. C. Nwokediuko, “Current trends in the Management of gastroesophageal reflux disease: a review,” ISRN Gastroenterology, vol. 2012, Article ID 391631, 11 pages, 2012.
- M. G. Patti, W. J. Gasper, P. M. Fisichella, I. Nipomnick, and F. Palazzo, “Gastroesophageal reflux disease and connective tissue disorders: pathophysiology and implications for treatment,” Journal of Gastrointestinal Surgery, vol. 12, no. 11, pp. 1900–1906, 2008.
- A. Ruigómez, M.-A. Wallander, S. Johansson, and L. A. G. Rodríguez, “Irritable bowel syndrome and gastroesophageal reflux disease in primary care: is there a link?” Digestive Diseases and Sciences, vol. 54, no. 5, pp. 1079–1086, 2009.
- H. Beaumont and G. Boeckxstaens, “Recent developments in esophageal motor disorders,” Current Opinion in Gastroenterology, vol. 23, no. 4, pp. 416–421, 2007.
- J. E. Pandolfino, Q. G. Zhang, S. K. Ghosh, A. Han, C. Boniquit, and P. J. Kahrilas, “Transient lower esophageal sphincter relaxations and reflux: mechanistic analysis using concurrent fluoroscopy and high-resolution manometry,” Gastroenterology, vol. 131, no. 6, pp. 1725–1733, 2006.
- S. Orenstein, J. Peters, S. Khan, N. Youssef, and S. Z. Hussain, “The esophagus,” in Nelson Tratado de Pediatría, R. M. Kliegman, R. E. Behrman, H. B. Jenson, and B. F. Stanton, Eds., p. 2427, W. B. Saunders Company, Madrid, Spain, 2004.
- C. S. Peter, C. Wiechers, B. Bohnhorst, J. Silny, and C. F. Poets, “Influence of nasogastric tubes on gastroesophageal reflux in preterm infants: a multiple intraluminal impedance study,” Journal of Pediatrics, vol. 141, no. 2, pp. 277–279, 2002.
- F. Cresi, L. De Sanctis, F. Savino, R. Bretto, A. Testa, and L. Silvestro, “Relationship between gastro-oesophageal reflux and gastric activity in newborns assessed by combined intraluminal impedance, pH metry and epigastric impedance,” Neurogastroenterology and Motility, vol. 18, no. 5, pp. 361–368, 2006.
- G. Davidson, “The role of lower esophageal sphincter function and dysmotility in gastroesophageal reflux in premature infants and in the first year of life,” Journal of Pediatric Gastroenterology and Nutrition, vol. 37, pp. S17–S22, 2003.
- H. Park, E. Clark, J. J. Cullen, J. G. Koland, M. S. Kim, and J. L. Conklin, “Expression of inducible nitric oxide synthase in the lower esophageal sphincter of the endotoxemic opossum,” Journal of Gastroenterology, vol. 37, no. 12, pp. 1000–1004, 2002.
- T. I. Omari, N. Rommel, E. Staunton et al., “Paradoxical impact of body positioning on gastroesophageal reflux and gastric emptying in the premature neonate,” Journal of Pediatrics, vol. 145, no. 2, pp. 194–200, 2004.
- Y. K. L. Koda, M. J. Ozaki, K. Murasca, and E. Vidolin, “Clinical features and prevalence of gastroesophageal reflux disease in infants attending a pediatric gastroenterology reference service,” Arquivos de Gastroenterologia, vol. 47, no. 1, pp. 66–71, 2010.
- M. P. Tighe, N. A. Afzal, A. Bevan, and R. M. Beattie, “Current pharmacological management of gastro-esophageal reflux in children: an evidence-based systematic review,” Pediatric Drugs, vol. 11, no. 3, pp. 185–202, 2009.
- A. A. Condino, J. Sondheimer, Z. Pan, J. Gralla, D. Perry, and J. A. O'Connor, “Evaluation of infantile acid and nonacid gastroesophageal reflux using combined pH monitoring and impedance measurement,” Journal of Pediatric Gastroenterology and Nutrition, vol. 42, no. 1, pp. 16–21, 2006.
- E. V. Guimarães, C. Marguet, and P. A. M. Camargos, “Treatment of gastroesophageal reflux disease,” Jornal de Pediatria, vol. 82, supplement 2, pp. S133–S145, 2006.
- H. A. Fornari, D. L. Nunes, and C. T. Ferreira, “Managing gastroesophageal reflux dsease in children: the role of endoscopy,” World Journal of Gastrointestinal Endoscopy, vol. 4, no. 8, pp. 339–346, 2012.
- T. G. Wenzl, “Evaluation of gastroesophageal reflux events in children using multichannel intraluminal electrical impedance,” American Journal of Medicine, vol. 115, no. 3, pp. 161–165, 2003.
- R. Rosen and S. Nurko, “The importance of multichannel intraluminal impedance in the evaluation of children with persistent respiratory symptoms,” American Journal of Gastroenterology, vol. 99, no. 12, pp. 2452–2458, 2004.
- B. D. Gold, “Review article: epidemiology and management of gasroesophageal reflux in children,” Alimentary Pharmacology and Therapeutics, vol. 19, pp. 22–27, 2004.
- J. Gil-Vernet and J. Boix-Ochoa, “Valor Clínico de la pH-metría intraesofágica en pediatría,” Anales Españoles de Pediatría, vol. 21, pp. 125–131, 1984.
- Y. Vandenplas and L. Sacre, “Continuous 24-hour esophageal pH monitoring in 285 asyntomatic infants 0-15 months old,” Journal of Pediatric Gastroenterology and Nutrition, vol. 6, pp. 220–224, 1987.
- J. Weigt, K. Mönkemüller, U. Peitz, and P. Malfertheiner, “Multichannel intraluminal impedance and pH-metry for investigation of symptomatic gastroesophageal reflux disease,” Digestive Diseases, vol. 25, no. 3, pp. 179–182, 2007.
- F. De la Morena, C. Santander, J. Cantero, T. Pérez, and R. Moreno, “Impedancia esofágica intraluminal de canal múltiple: una nueva frontera en motilidad,” Revista Española de Enfermedades Digestivas, vol. 100, pp. 86–89, 2008.
- D. Sifrim, D. Castell, J. Dent, and P. J. Kahrilas, “Gastro-oesophageal reflux monitoring: review and consensus report on detection and definitions of acid, non-acid, and gas reflux,” Gut, vol. 53, no. 7, pp. 1024–1031, 2004.
- A. Ruiz de León and J. A. Pérez de la Serna, “Impedancia intraluminal multicanal asociada a pHmetría en el studio de la enfermedad por reflujo gastroesofágico,” Revista Española de Enfermedades Digestivas, vol. 100, no. 2, pp. 67–70, 2008.
- A. Agrawal and D. Castell, “Clinical importante of impedance measurements,” Journal of Clinical Gastroenterology, vol. 42, pp. 579–583, 2008.
- C. M. Loots, M. A. Benninga, G. P. Davidson, and T. I. Omari, “Addition of pH-Impedance monitoring to standard pH monitoring increases the yield of symptom association analysis in infants and children with gastroesophageal reflux,” Journal of Pediatrics, vol. 154, no. 2, pp. 248–252, 2009.
- D. O. Castell and M. Vela, “Combined multichannel intraluminal impedance and pH-metry: an evolving technique to measure type and proximal extent of gastroesophageal reflux,” American Journal of Medicine, vol. 111, no. 8, pp. 157–159, 2001.
- F. Zerbib, S. Bruley Des Varannes, S. Roman et al., “Normal values and day-to-day variability of 24-h ambulatory oesophageal impedance-pH monitoring in a Belgian-French cohort of healthy subjects,” Alimentary Pharmacology and Therapeutics, vol. 22, no. 10, pp. 1011–1021, 2005.
- S. Shay, R. Tutuian, D. Sifrim et al., “Twenty-four hour ambulatory simultaneous impedance and pH monitoring: a multicenter report of normal values from 60 healthy volunteers,” American Journal of Gastroenterology, vol. 99, no. 6, pp. 1037–1043, 2004.
- M. Marzo, P. Alonso, X. Bonfill, et al., “Guía práctica clínica sobre el manejo del paciente con enfermedad por reflujo gastroesofágico (ERGE),” Journal of Gastroenterology and Hepatology, vol. 25, pp. 85–110, 2002.
- T. G. Wenzl, S. Schneider, F. Scheele, J. Silny, G. Heimann, and H. Skopnik, “Effects of thickened feeding on gastroesophageal reflux in infants: a placebo-controlled crossover study using intraluminal impedance,” Pediatrics, vol. 111, no. 4, pp. e355–e359, 2003.
- L. Corvaglia, M. Ferlini, R. Rotatori, A. Aceti, and G. Faldella, “Body position and gastroesophageal reflux in premature neonates: evaluation by combined pH-impedance monitoring,” Journal of Pediatric Gastroenterology and Nutrition, vol. 42, pp. 50–51, 2006.
- N. Øyen, T. Markestad, R. Skjærven et al., “Combined effects of sleeping position and prenatal risk factors in sudden infant death syndrome: the nordic epidemiological SIDS study,” Pediatrics, vol. 100, no. 4, pp. 613–621, 1997.
- B. Bagucka, J. De Schepper, M. Peelman, K. Van De Maele, and Y. Vandenplas, “Acid gastro-esophageal reflux in the 10°-reversed-Trendelenburg- position in supine sleeping infants,” Acta Paediatrica Taiwanica, vol. 40, no. 5, pp. 298–301, 1999.
- C. D. Rudolph, L. J. Mazur, G. S. Liptak et al., “Guidelines for evaluation and treatment of gastroesophageal reflux in infants and children: recommendations of the North America Society for Pediatric Gastroenterology and Nutrition,” Journal of Pediatric Gastroenterology and Nutrition, vol. 32, supplement 2, pp. S1–S31, 2001.
- S. M. Grant, H. D. Langtry, and R. N. Brogden, “Ranitidine. An updated review of its pharmacodynamic and pharmacokinetic properties and therapeutic use in peptic ulcer disease and other allied diseases,” Drugs, vol. 37, no. 6, pp. 801–870, 1989.
- G. Der, “An overview of proton pump inhibitors,” Gastroenterology Nursing, vol. 26, pp. 182–190, 2003.
- M. Robinson and J. Horn, “Clinical Pharmacology of proton pump inhibitors. What the practising physician needs to know,” Drugs, vol. 63, no. 24, pp. 2739–2754, 2003.
- B. T. Vanderhoff and R. M. Tahboub, “Proton pump inhibitors, an update,” The Journal of Clinical Pharmacology, vol. 66, pp. 273–280, 2002.
- S. Shi and U. Klotz, “Proton pump inhibitors, an update of their clinical use and pharmacokinetics,” European Journal of Clinical Pharmacology, vol. 64, pp. 935–951.
- V. Savarino, F. Di Mario, and C. Scarpignato, “Proton pump inhibitors in GORD. An overview of their pharmacology, efficacy and safety,” Pharmacological Research, vol. 59, no. 3, pp. 135–153, 2009.
- T. E. Gibbons and B. D. Gold, “The use of proton pump inhibitors in children: a comprehensive review,” Pediatric Drugs, vol. 5, no. 1, pp. 25–40, 2003.
- N. Chiba, C. J. De Gara, J. M. Wilkinson, and R. H. Hunt, “Speed of healing and symptom relief in grade II to IV gastroesophageal reflux disease: a meta-analysis,” Gastroenterology, vol. 112, no. 6, pp. 1798–1810, 1997.
- E. Hassall, D. Israel, R. Shepherd et al., “Omeprazole for treatment of chronic erosive esophagitis in children: a multicenter study of efficacy, safety, tolerability and close requirements,” Journal of Pediatrics, vol. 137, no. 6, pp. 800–807, 2000.
- D. A. Gremse, “GERD in the pediatric patient: management considerations,” MedGenMed Medscape General Medicine, vol. 6, no. 2, article 13, 2004.
- M. Karjoo and R. Kane, “Omeprazole treatment of children with peptic esophagitis refractory to ranitidine therapy,” Archives of Pediatrics and Adolescent Medicine, vol. 149, no. 3, pp. 267–271, 1995.
- S. Cucchiara, R. Minella, C. Iervolino et al., “Omeprazole and high dose ranitidine in the treatment of refractory reflux oesophagitis,” Archives of Disease in Childhood, vol. 69, no. 6, pp. 655–659, 1993.
- L. Rinaman, J. P. Card, J. S. Schwaber, and R. R. Miselis, “Ultrastructural demonstration of a gastric monosynaptic vagal circuit in the nucleus of the solitary tract in rat,” Journal of Neuroscience, vol. 9, no. 6, pp. 1985–1996, 1989.
- A. J. Page and L. A. Blackshaw, “GABA(B) receptors inhibit mechanosensitivity of primary afferent endings,” Journal of Neuroscience, vol. 19, no. 19, pp. 8597–8602, 1999.
- T. I. Omari, M. A. Benninga, L. Sansom, R. N. Butler, J. Dent, and G. P. Davidson, “Effect of baclofen on esophagogastric motility and gastroesophageal reflux in children with gastroesophageal reflux disease: a randomized controlled trial,” Journal of Pediatrics, vol. 149, no. 4, pp. 468–474.e2, 2006.
- M. Kawai, H. Kawahara, S. Hirayama, N. Yoshimura, and S. Ida, “Effect of baclofen on emesis and 24-hour esophageal pH in neurologically impaired children with gastroesophageal reflux disease,” Journal of Pediatric Gastroenterology and Nutrition, vol. 38, no. 3, pp. 317–323, 2004.
- N. Vakil, “Review article: new pharmacological agents for the treatment of gastro-oesophageal reflux disease,” Alimentary Pharmacology and Therapeutics, vol. 19, no. 10, pp. 1041–1049, 2004.
- G. Coruzzi, E. Poli, G. Morini, and G. Bertaccini, “The histamine H3 receptor,” in Molecular Targets for Drug Development: GI Diseases, T. S. Gaginela and A. Guglietta, Eds., pp. 239–267, Humana Press, Totowa, NJ, USA, 1st edition, 2000.
- Anonymous, “Gastrin 17 vaccine-Aphton: anti-gastrin 17 inmunogen,G17DT,” BioDrugs, vol. 17, pp. 223–225, 2003.
- B. D. Gold, “Asthma and gastroesophageal reflux disease in children: exploring the relationship,” Journal of Pediatrics, vol. 146, no. 3, pp. S13–S20, 2005.
- S. Somme, J. A. Rodriguez, D. G. Kirsch, and D. C. Liu, “Laparoscopic versus open funduplication in infants,” Surgical Endoscopy, vol. 16, pp. 54–56, 2002.
- M. Saedon, S. Gourgiotis, and S. Germanos, “Is there a changing trend in surgical management of gastroesophageal reflux disease in children?” World Journal of Gastroenterology, vol. 13, no. 33, pp. 4417–4422, 2007.
- C. Di Lorenzo and S. Orenstein, “Fundoplication:friend or foe?” Journal of Pediatric Gastroenterology and Nutrition, vol. 34, pp. 117–124, 2002.
- G. Nind, W. H. Chen, R. Protheroe et al., “Mechanisms of gastroesophageal reflux in critically ill mechanically ventilated patients,” Gastroenterology, vol. 128, no. 3, pp. 600–606, 2005.
- T. A. Abdel-Gawad, M. A. El-Hodhod, H. M. Ibrahim, and Y. W. Michael, “Gastroesophageal reflux in mechanically ventilated pediatric patients and its relation to ventilator-associated pneumonia,” Critical Care, vol. 13, no. 5, article R164, 2009.
- N. A. G. Hak, M. Mostafa, T. Salah, M. El-Hemaly, M. H. A. A. El-Raouf, and E. Hamdy, “Acid and bile reflux in erosive reflux disease, non-erosive reflux disease and Barrett's esophagus,” Hepato-Gastroenterology, vol. 55, no. 82-83, pp. 442–447, 2008.
- J. Ibanez, A. Penafiel, P. Marse, R. Jorda, J. M. Raurich, and F. Mata, “Incidence of gastroesophageal reflux and aspiration in mechanically ventilated patients using small-bore nasogastric tubes,” Journal of Parenteral and Enteral Nutrition, vol. 24, no. 2, pp. 103–106, 2000.
- Y. Xin, N. Dai, L. Zhao, J. G. Wang, and J. M. Si, “The effect of famotidine on gastroesophageal and duodeno-gastroesophageal refluyes in critically ill patients,” World Journal of Gastroenterology, vol. 9, no. 2, pp. 356–358, 2003.
- C. H. Kao, S. P. Changlai, P. U. Chieng, and T. C. Yen, “Gastric emptying in head-injured patients,” American Journal of Gastroenterology, vol. 93, no. 7, pp. 1108–1112, 1998.
- F. W. Woodley and H. Mousa, “Acid gastroesophageal reflux reports in infants: a comparison of esophageal pH monitoring and multichannel intraluminal impedance measurements,” Digestive Diseases and Sciences, vol. 51, no. 11, pp. 1910–1916, 2006.
- A. Wilmer, J. Tack, E. Frans et al., “Duodenogastroesophageal reflux and esophageal mucosal injury in mechanically ventilated patients,” Gastroenterology, vol. 116, no. 6, pp. 1293–1299, 1999.
- J. C. Montejo, J. Jiménez, J. Ordóñez, et al., “Complicaciones gastrointestinales de la nutrición enteral en el paciente crítico,” Revista Medicina Intensiva, vol. 25, pp. 152–160, 2001.
- J. López-Herce, “Gastrointestinal complications in critically ill patients: what differs between adults and children?” Current Opinion in Clinical Nutrition and Metabolic Care, vol. 12, no. 3, pp. 180–185, 2009.
- G. P. Zaloga and P. Marik, “Promotility agents in the intensive care unit,” Critical Care Medicine, vol. 28, pp. 2657–2658, 2000.
- M. F. Vela, L. Camacho-Lobato, R. Srinivasan, R. Tutuian, P. O. Katz, and D. O. Castell, “Simultaneous intraesophageal impedance and pH measurement of acid and nonacid gastroesophageal reflux: effect of omeprazole,” Gastroenterology, vol. 120, no. 7, pp. 1599–1606, 2001.